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Purified anti-Tollip Antibody

Pricing & Availability
Clone
O92A4 (See other available formats)
Regulatory Status
RUO
Other Names
Tollip, Toll Interacting Protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
O92A4_Pure_Tollip_Antibody_1_082718
Western blot of purified anti-Tollip antibody (clone O9I2A4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O92A4_Pure_Tollip_Antibody_1_082718
    Western blot of purified anti-Tollip antibody (clone O9I2A4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Save
854401 25 µg ¥23,320
854402 100 µg ¥60,720
Description

Tollip, also known as Toll interacting protein, is a ubiquitin-binding protein and an inhibitory adaptor protein involved  in IL-1 and Toll-like receptor (TLR) signaling pathway.  Alterations in Tollip gene expression levels has been linked to  tuberculosis and idiopathic pulmonary fibrosis, suggesting a role for Tollip in innate immune system. Tollip has also been reported to mediate autophagic clearance of poly-ubiquitinated proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human recombinant Tollip (NP_061882) produced in HEK293T cell.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is highly specific to human Tollip. It does not cross react with murine Tollip.

RRID
AB_2750472 (BioLegend Cat. No. 854401)
AB_2750472 (BioLegend Cat. No. 854402)

Antigen Details

Structure
Tollip is a 274 amino acid protein with an apparent molecular mass of ~30 kD.
Distribution

Tissue Distribution: Ubiquitously expressed.
Cellular Distribution: Plasma membrane, extracellular, cytosol, lysosome, and nucleus.

Function
Tollip is a component of the IL-1 and TLR signaling pathway involved in innate immune response.
Interaction
Tollip has been reported to interact with TOM1, TLR2, TLR4, IRAK1, IR-1RI, TOM1L2, and ILRAP.
Biology Area
Cell Biology, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Enzymes and Regulators
Antigen References
  1. Capelluto DG. 2012. Microbes Infect. 14(2): 140
  2. Brandao I, et al. 2015. Gut Microbes. 6(3):202
Gene ID
54472 View all products for this Gene ID
UniProt
View information about Tollip on UniProt.org
Go To Top Version: 1    Revision Date: 08/27/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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