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- Clone
- P82D6D7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α-tubulin 8, Tubulin α Chain-Like 2, TUBAL2, TUBA8
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total cell lysate from HeLa (lane 1, 15 µg), MCF-7 (lane 2, 15 µg) and Jurkat (lane 3, 15 µg) were resolved by electrophoresis (4-12 % Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-Tubulin α 8 antibody (clone P82D6D7). Proteins were visualized using a HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection. Purified anti-β-actin antibody (clone Poly6221) was used as a loading control. -
HeLa cells were fixed with 4% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti-Tubulin α 8 (clone P82D6D7) in blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta tubulin. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. Tubulin α 8 belongs to the Tubulin α family which contains 8 other members such as tubulin α 1A, tubulin α 4A, tubulin α 3E. It has been shown that mutations in the tubulin α 8 gene is associated with polymicrogyria and optic nerve hypoplasia.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified recombinant fragment of human Tubulin α 8 (a.a. 294-449) expressed in E. coli. This region shares high percentage of homology with other Tubulin α members.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone P82D6D7 may also recognize the other Tubulin a members since this clone was developed with partial sequence of tubulin a 8 (a.a. 294-449) that shares high percentage of homology with other members in the Tubulin a family such as tubulin a 4A, tubulin a 3C/D, and tubulin a 1A.
- RRID
-
AB_2632654 (BioLegend Cat. No. 691702)
Antigen Details
- Structure
- Tubulin α 8 contains 449 amino acids with a predicted molecular weight of 50 kD.
- Distribution
-
Cytoskeleton/Ubiquitous.
- Function
- Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site.
- Interaction
- RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell. Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.
4. Abdollahi MR, et al. 2009. Am. J. Hum. Genet. 85:737. - Gene ID
- 51807 View all products for this Gene ID
- UniProt
- View information about Tubulin alpha 8 on UniProt.org
Other Formats
View All Tubulin α 8 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Tubulin α 8 | P82D6D7 | WB,ICC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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