- Clone
- 7B9.Rec (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tubulin beta-2B chain, class IIb beta-tubulin, class II beta-tubulin isotype, tubulin, beta polypeptide paralog
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
637551 | 25 µg | ¥38,500 | |
637552 | 100 µg | ¥94,600 |
Tubulin is a major component of microtubules. It exists as a heterodimer consisting of an alpha and beta subunit. Class II beta tubulin is the major form of tubulin beta in neurons and is found in many other tissues including lung tissue and Schwann cells. TUBB2B is also indicated in various cancers and has been shown to have increased expression in hepatocellular carcinomas.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Recombinant peptide of Tubulin-B 2B
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For the use of this antibody (clone 7B9.rec) in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, pH 9.0) or Citrate Buffer, 10X (Cat. No. 420902).
For the use of this antibody (clone 7B9.rec) in immunocytochemistry (ICC), it is recommended to perform fixation/permeabilization with either 100% ice-cold methanol or fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 100% ice-cold methanol. - RRID
-
AB_3662318 (BioLegend Cat. No. 637551)
AB_3662318 (BioLegend Cat. No. 637552)
Antigen Details
- Structure
- TUBB2B is a 455 amino acid protein with a predicted molecular weight of 50 kD.
- Distribution
-
Cytoplasm, cytoskeleton, and microtubules
- Function
- Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
- Moody SA, et al. 1996. J Comp Neuro. 364: 219-30.
- Dozier JH, et al. 2003. Breast Cancer Res. 5:R157-69.
- Gene ID
- 347733 View all products for this Gene ID
- UniProt
- View information about Tubulin-β on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Tubulin-β Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tubulin-β 2B (TUBB2B) Recombinant Antibody | 7B9.Rec | WB,IHC-P,ICC |
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Purified anti-Tubulin-β 2B (TUBB2B) Recombinant Antibody
Whole cell extracts (15 µg total protein) from the indicated... Whole cell extracts (15 µg total protein) from the indicated... IHC staining of Purified anti-TUBB2B (clone 7B9.rec) on form... HeLa cells (negative cell line, panel A) and U2-OS cells (po... HeLa cells (negative cell line, panel A) and U2-OS cells (po...
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