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- Clone
- 14C11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- γ-tubulin
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total lysates (15 µg protein) from HaCaT, HeLa (Human) and Raw264.7 (Mouse) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg/ml) purified anti-Tubulin-γ antibody, clone 14C11. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat.No. 643807, 1:8000 dilution). -
Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against g-Tubulin (clone 14C11) followed by Rhodamine Red-X conjugated donkey anti-mouse IgG and DAPI.
γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Peptide-KLH
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:500-1:1000). For immunocytochemistry, a concentration of 2.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The purified 14C11 antibody has been shown to be useful for Western blotting of human, mouse, and rat ?-tubulin.
- Product Citations
-
- RRID
-
AB_493419 (BioLegend Cat. No. 629201)
Antigen Details
- Structure
- Tubulin superfamily, highly conserved between yeast and mammals. Predicted molecular weight approximately 51 kD
- Distribution
-
Cytoskeletal protein, high expressed in centrosomes
- Function
- Cytoskeletal organization, mitosis
- Interaction
- Interacts with α-tubulin, paxillin, RAC GTPase activated protein 1
- Modification
- Phosphorylation
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
1. Zheng Y, et al. 1991. Cell 65:817.
- Regulation
- Expressed at highest levels during prophase to metaphase
- Gene ID
- 2776 View all products for this Gene ID
- UniProt
- View information about Tubulin-gamma on UniProt.org
Other Formats
View All Tubulin-γ Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Tubulin-γ | 14C11 | WB,ICC |
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