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Purified anti-VEGF Antibody

Pricing & Availability
Clone
Poly6275 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial growth factor, vasculotropin, vascular permeability factor
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
VEGF_090105
Human recombinant VEGF 165 produced in yeast was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-VEGF polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody detects reduced VEGF consistent with the molecular weight shown (approximately 22-24 kD) as well as unreduced VEGF (data not shown).
  • VEGF_090105
    Human recombinant VEGF 165 produced in yeast was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-VEGF polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody detects reduced VEGF consistent with the molecular weight shown (approximately 22-24 kD) as well as unreduced VEGF (data not shown).
Cat # Size Price Save
627501 50 µL ¥25,960
Description

VEGF, also known as vascular endothelial growth factor, vasculotropin, and vascular permeability factor, is a widely expressed mitogen for vascular endothelial cells that has been shown to promote angiogenesis and increase permeability of capillary blood vessels. VEGF is a member of the platelet-derived growth factor (PDGF) family and is produced as a homodimeric protein with approximate molecular weights of 34-46 kD. The variance in molecular weight is a result of alternative splicing events that encode monomeric proteins having 121, 165, 189 or 206 amino acids. VEGF121 and VEGF165 are secreted proteins whereas VEGF189 and VEGF206 are strongly cell-associated. VEGF binds to VEGF-R1 (also known as Flt-1) and VEGF-R2 (also known as KDR). Inhibition of VEGF biding to VEGF-Rs has been the focus of a number of tumor therapeutic strategies. The Poly6275 antibody recognizes the N-terminal region of human VEGF has been shown to be useful for Western blotting of both reduced and non-reduced VEGF.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide mapping to a domain near the N-terminus
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Additional Product Notes
The 50 µL size can be used for approximately 5 Western blots.
Product Citations
  1. Calvo-López T, et al. 2023. Front Microbiol. 13:1063706. PubMed
  2. Liang W, et al. 2021. Oxid Med Cell Longev. 2021:5537737. PubMed
  3. Jin G, et al. 2020. Oxid Med Cell Longev. 7690845:2020. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 627501)

Antigen Details

Structure
Contains 8 cysteine residues involved in intra- and inter-chain disulfide bonds, platelet-derived growth factor (PDGF) family, produced as a homodimeric protein with an approximate molecular weight of 34-46 kD. Human VEGF is alternatively spliced to encod
Distribution

Widely expressed

Function
Mitogen for vascular endothelial cells, promotes angiogenesis and increases permeability of capillary blood vessels
Interaction
Heparin, many other proteins
Ligand/Receptor
VEGF-R1 (Flt-1) and VEGF-R2 (KDR)
Modification
Glycosylation
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Connolly DT, et al. 1989. J. Clin. Invest. 84:1470.
2. Drake CJ, et al. 1995. Proc. Natl. Acad. Sci. USA 92:7657.
3. Houck KA, et al. 1991. Mol. Endocrinol. 5:1806.

Gene ID
7422 View all products for this Gene ID
UniProt
View information about VEGF on UniProt.org
Go To Top Version: 3    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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