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Purified anti-VPS35 Antibody

Pricing & Availability
Clone
A17084A (See other available formats)
Regulatory Status
RUO
Other Names
Vacuolar protein sorting-associated protein 35
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A17084A_Pure_VPS35_Antibody_1_102418
Western blot of purified anti-VPS35 antibody (clone A17084A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of HEK293 lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17084A_Pure_VPS35_Antibody_1_102418
    Western blot of purified anti-VPS35 antibody (clone A17084A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of HEK293 lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17084A_Pure_VPS35_Antibody_2_102418
    Western blot of purified anti-VPS35 antibody (clone A17084A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 ng of recombinant human VPS35 protein. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Save
856401 25 µg ¥24,640
856402 100 µg ¥63,360
Description

VPS35 is the largest subunit of the retromer complex, which plays an important role in retrograde protein transport from the endosome to the trans-Golgi network. VPS35 binds to VPS26 (VPS26A or VPS26B) and VPS29 to form the vacuolar protein sorting subcomplex (VPS), which in turn interacts with different nexins to form different retromer variants that transport different targets. D620N mutation in VPS35 has been implicated as a causative genetic factor for developing late-onset, autosomal dominant familial Parkinson’s disease, and could serve as potential therapeutic target for PD.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783456 (BioLegend Cat. No. 856401)
AB_2783456 (BioLegend Cat. No. 856402)

Antigen Details

Structure
VPS35 is a 796 amino acid protein with an apparent molecular mass of ~92 kD.
Distribution

Tissue Distribution: Ubiquitously expressed in all tissues.
Cellular Distribution: Cytosol, lysosome, endosome, and secreted.

Function
Largest subunit of the retromer complex involved in retrograde transport of proteins from endosomes to the trans-Golgi network.
Interaction
VPS26 (VPS26A or VPS26B), VPS29, SNX1, SNX2, IGF2R, SNX3, GOLPH3, LRRK2, SLC11A2, SNX27, RAB7A.
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Organelle Markers
Antigen References
  1. Cui Y, et al. 2018. FEBS Lett. 592(7):1096
  2. Williams ET, et al. 2017. J Parkinsons dis. 7(2):219
  3. Ferreira M, et al. 2017. Acta Neurol Scand. 135(3):273
Gene ID
55737 View all products for this Gene ID 65114 View all products for this Gene ID 25479 View all products for this Gene ID
UniProt
View information about VPS35 on UniProt.org
Go To Top Version: 1    Revision Date: 10/24/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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