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- Regulatory Status
- RUO
- Other Names
- B-cell stimulatory factor 2, Interferon beta-2, Hybrid¬oma growth factor, CTL differentiation factor, B-cell differentiation factor, BSF2, BSF-2, CDF, HSF, IFNB2, IFN-beta-2, IL6, IL-6, interleukin 6, interleukin BSF-2, interleukin-6, MGI-2A
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Human IL-6 (also known as B-cell stimulatory factor 2, Interferon beta-2, Hy¬bridoma growth factor, and CTL differentiation factor) plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth, nerve cells differentiation, and acute phase reac¬tants in hepatocytes. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. It has been shown that IL-6 plays a critical role in many physiological and pathological conditions, including autoimmune diseases, such as rheumatoid arthritis.
The BioLegend RAPID MAX™ Human IL-6 ELISA Kit is a one step, one wash, 80 minute Enzyme-Linked Immunosorbent Sandwich Assay (ELISA) with a pre-coated, 96-well strip plate that binds the biotinylated capture antibody. Standards and samples are added into the wells, followed by an antibody cocktail. The antibody cocktail consists of an biotinylated rat monoclonal capture antibody and an HRP-conjugated rat monoclonal detection antibody. This kit is specifically designed for the accurate quantitation of human IL-6 from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
Kit Contents
- Kit Contents
-
- RAPID MAX™ Pre-coated 96-well Strip Microplate
- Human IL-6 Detection Antibody Concentrate (200X)
- Human IL-6 Capture Antibody Concentrate (200X)
- Human IL-6 Standard
- Assay Diluent A (5X)
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 5.99 pg/mL
- Standard Range
- 15.6-1000 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 μL to 1,000 μL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Phosphate-Buffered Saline (PBS)
Antigen Details
- Biology Area
- Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 3569 View all products for this Gene ID
- UniProt
- View information about IL-6 on UniProt.org
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