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- Regulatory Status
- RUO
- Other Names
- BAG-3, BIS, BAG Cochaperone 3, BAG Family Molecular ChaperoneRegulator 3, BCL2 Associated Athanogene 3, Bcl-2-Binding ProteinBis Transcript 2, CAIR-1, MFM6, Docking Protein CAIR-1, BCL2-Binding Athanogene 3
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- Submit a Review
- Product Citations
- publications
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Stability Testing for Recombinant Human BAG3. One aliquot (Control) was stored at 4°C for 7 days, while another aliquot (4XF/T) was subjected to multiple freeze-thaw cycles by freezing at -80°C and thawing four times over a 7-day period. Each freeze-thaw cycle involved placing the sample on ice and slowly thawing it until no ice crystals remained in the tube. A minimum of 24 hours elapsed between each freeze and thaw event. Following treatment, the reduced samples (4 µg/lane) were separated by SDS-PAGE and stained with Coomassie brilliant blue dye. Lane M: Protein ladder (Cat. No. 773302).
Bcl2-associated athanogene 3 (BAG3) is a member of BAG family of co-chaperones and multifunctional protein. BAG3 supports cellular homeostasis by regulating autophagy, cell structure, and cellular integrity. BAG3 is a multi-domain scaffolding protein composed of a WW domain, two isoleucine–proline–valine (IPV) motifs, a proline-rich repeat (PxxP) region, and a BAG domain. The BAG domains of BAG1, BAG2, and BAG3 interact with the Hsc70 ATPase domain. BAG3 also binds to 14-3-3 proteins and the ATPase motif of heat shock protein70 (Hsp70). BAG3 associates with many small heat shock proteins such as HspB1, HspB5, HspB6, and HspB8. BAG3 is ubiquitously expressed with higher expression observed in skeletal muscles, heart, central nervous systems, placenta, and various types of tumor cells. Studies have demonstrated that BAG3 protein levels are lower in human Alzheimer’s disease brains compared to control groups. Overexpression of BAG3 promotes the degradation of misfolded proteins in neurodegeneration. BAG3 plays a critical role in neuronal proteostasis and pathogenesis of neurodegenerative diseases.
Product DetailsProduct Details
- Source
- Human BAG3, amino acids (Met1-Pro575) (Accession #O95817) with a C-terminal 6His tag was expressed in insect cells.
- Molecular Mass
- The 585 amino acid recombinant protein has a predicted molecular mass of approximately 62.7 kD. The DTT-reduced and non-reduced protein migrates at approximately 62-80 kD by SDS-PAGE. The N-terminal amino acid is Met.
- Purity
- > 85% as determined by Coomassie stained SDS-PAGE.
- Formulation
- Protein is formulated in 20 mM Tris, 1 mM EDTA, 0.1 mM PMSF, 10% Glycerol, pH 8.0
- Concentration
- 100 µg at 0.5 mg/mL
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 1 week, at -20°C for up to three months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Application
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Standard
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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BAG3 is highly expressed in skeletal muscle, cardiomyocytes and multiple tumors
- Function
- BAG3 supports the diverse functions of the different cell types, including cancer cells, myocytes, and neurons
- Interaction
- Hsp70, HspB1, HspB5, HspB6, and HspB8
- Cell Type
- Endothelial cells, Neurons
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immuno-Oncology, Neurodegeneration, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Enzymes and Regulators
- Antigen References
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- Brenner CM, et al. 2023. Cells. 12:937.
- Lin H, et al. 2023. Neuroscience. 518:4-9.
- Sheehan PW, et al. 2023. Neuron. 111:2383-2398.e7
- Klimek C, et al. 2017. J Cell Sci. 130:2781-2788.
- Fang X, et al. 2019. JCI Insight. 4:e126464.
- Hiebel C, et al. 2020. Cells. 9:2416.
- Sarah S, et al. 2018. Oncotarget. 9:15673.
- Kirk JA, et al. 20121. OJ Clin Investig. 131:1.
- Behl C. 2011. Autophagy. 7:795-8.
- Gamerdinger M, et al. 2011. J Mol Med. 89:1175-82.
- Zhou J, et al. 2020. Biol Psychiatry. 87:756-769.
- Chen Y, et al. 2013. Mol Cell Proteomics. 12:2804-19.
- Gene ID
- 9531 View all products for this Gene ID
- UniProt
- View information about BAG3 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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