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Recombinant Human CSF-1R (CD115)-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Macrophage colony-stimulating factor 1 receptor, M-CSFR, M-CSF-R, CSF-1 receptor, CSF-1R, CSF-1-R, CSF1R, Proto-oncogene c-Fms, FMS, CSF-1R, CSF-1/CSF-1R
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Product Citations
publications
Human_CD115-_CF_1_121622
Human CSF-1R (CD115) inhibits the proliferation of M-NFS-60 cells induced by recombinant human M-CSF (Cat. No. 574802) at 1 ng/mL. The ED50 for this effect is 4 – 20 ng/mL.
  • Human_CD115-_CF_1_121622
    Human CSF-1R (CD115) inhibits the proliferation of M-NFS-60 cells induced by recombinant human M-CSF (Cat. No. 574802) at 1 ng/mL. The ED50 for this effect is 4 – 20 ng/mL.
  • Human_CD115-_CF_2_121622
    Stability Testing for Recombinant Human CSF-1R (CD115)-Fc Chimera. Recombinant Human CSF-1R (CD115)-Fc Chimera was aliquoted in PBS at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to inhibit the proliferation of M-NFS-60 cells induced by M-CSF (Cat. No. 574802) at 1 ng/mL. The ED50 for this effect is 4 – 20 ng/mL.
Cat # Size Price Save
798504 25 µg ¥30,850
798506 100 µg ¥79,600

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CSF-1R, also known as CD115 and M-CSFR, is a member of the CSF-1/PDGF (platelet-derived growth factor) receptor family.  It consists of five Ig-like C2 type domains and a split tyrosine kinase domain on its extracellular portion, an intracellular domain, and a C-terminal tail. CSF-1R is expressed on monocytes/macrophages, plasmacytoid, conventional dendritic cells, and osteoclasts. It is a proto-oncogene, upregulated in several cancers including breast, ovarian, prostate, and endometrial cancer. Glucocorticoids regulates CSF-1R expression in breast cancer cells and endometrial cells. Its natural ligands, M-CSF and IL-34, lead to homodimerization and activation of CSF-1R by autophosphorylation and ubiquitination on its intracellular residues. CSF-1R is involved in regulation of survival, proliferation and differentiation of hematopoietic progenitor cells. CSF-1/CSF-1R plays an important role in innate immunity and inflammation by modulating cytotoxicity and cytokine production on monocyte/ macrophages. CSF-1/CSF-1R also has roles in differentiation of dendritic cells, osteoclasts and bone development. CSF-1R-deficient mice showed reduced mononuclear phagocyte production, low number of osteoclasts, increased splenic erythroid burst-forming unit (BFU-Es) and abnormal skeletal development. Constitutive CSF-1R activation promotes cadherin internalization, disrupts epithelial cell-cell adhesion and alters acinar morphogenesis. The shedding of CSF-1R is mediated by pro-TNF-α-converting enzyme (TACE) upon TPA and LPS treatment, resulting in CSF-1R down-modulation in activated microphages. Several studies demonstrate that CSF-1R inhibitors have protective effects on tumor progression and metastasis, indicating that CSF-1R is a potential target for cancer therapy. In addition, several mutations on csf-1r gene were identified and associate with a progressive neurodegenerative disorder, hereditary diffuse leukoencephalopathy with axonal spheroids (HDLS).

Product Details
Technical data sheet

Product Details

Source
Human CD115, amino acid Ile20-Glu512 (accession #CAA27300.1), with a linker (GSSR) and a C-terminal human IgG (Pro100-Lys330) and 6His tag, was expressed in CHO cells
Molecular Mass
The 734 amino acid recombinant protein has a predicted molecular mass of approximately 81.63 kD. The DTT-reduced and non-reduced protein migrates at approximately 120 kD and 240 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ile.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human CD115 inhibits the proliferation of M-NFS-60 cells induced by recombinant human M-CSF (Cat. No. 574802) at 1 ng/mL. The ED50 for this effect is 4 – 20 ng/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Primitive multipotent hematopoietic cells, monocytes/macrophages, plasmacytoid, conventional dendritic cells, B cells, smooth muscle cells, neuron, osteoclasts and some cancer cells; plasma membrane

Function
CD115 is involved in regulation of survival, proliferation and differentiation of hematopoietic progenitor cells. It is also involved in innate immunity and inflammation.
Ligand/Receptor
Macrophage Colony Stimulating Factor (M-CSF) and IL-34.
Bioactivity
Measured by its ability to bind recombinant human GFRα1
Cell Type
Dendritic cells, Epithelial cells, Hematopoietic stem and progenitors, Langerhans cells, Macrophages, Microglia, Monocytes, Neural Stem Cells, Neurons, Osteoclasts
Biology Area
Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroinflammation
Molecular Family
CD Molecules, Fc Receptors, Innate Immune Signaling, Soluble Receptors
Antigen References
  1. Sapi E, et al. 1995. Oncogene. 10:529-42.
  2. Dai XM, et al. 2002. Blood. 99:111-20.
  3. Lin EY, et al. 2001. J Exp Med. 193:727-40.
  4. Gill K, et al. 2001. Fertil Steril, 76:1005-11.
  5. Flick MB, et al. 2002. J Cell Biochem. 85:10-23.
  6. Chihara T, et al. 2010. Cell Death Differ. 17:1917-27. 
  7. Chitu V, et al. 2006. Curr Opin Immunol. 18:39-48. 
  8. Wei S, et al. 2010. J Leukoc Biol. 88:495-505. 
  9. Yeung YG, et al. 1998. J Biol Chem. 273:17128-37. 
  10. Chambers SK, et al. 1994. Steroids. 59:514-22 
  11. Ide H, et al. 2002. Proc Natl Acad Sci USA. 99:14404-9.
  12. Ohno H, et al. 2006. Mol Cancer Ther. 5:2634-43. 
  13. Rovida E, et al. 2001. J Immunol. 166:1583-9. 
  14. Wrobel CN, et al. 2004. J Cell Biol. 165:263-73.
  15. Cannarile MA, et al. 2017. J Immunother Cancer. 5:53. 
  16. Guo J, et al. 2006. Mol Cancer Ther. 5:1007-13. 
  17. Hiraga T, et al. 2009. Int J Cancer. 124:215-22.
  18. Machkani B, et al. 2010. Bioorg Med Chem. 18:1789-97. 
  19. Battisti C, et al. 2014. J Neurol. 261:768-72.
Gene ID
1436 View all products for this Gene ID
UniProt
View information about CSF-1R on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 12/16/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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