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- Regulatory Status
- RUO
- Other Names
- CD48 antigen, B-lymphocyte activation marker BLAST-1, Blast-1, SLAMF2, HuLy-m3, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1
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- Submit a Review
- Product Citations
- publications
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When human CD48-Fc Chimera is immobilized at 5 µg/mL, biotinylated human SLAMF4-Fc Chimera binds with EC50 of 0.75 - 3 µg/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding. -
Stability testing for human CD48-Fc Chimera. Recombinant human CD48-Fc Chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with biotinylated human SLAMF4-Fc Chimera.
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CD48, also known as SLAMF2, is one of the members of the CD2 subfamily. CD48 is a GPI-anchored membrane protein containing two Ig-like C2 type domains and is widely expressed on both resting and activated hematopoietic cells with the exception of granulocytes, platelets, and erythrocytes. CD48 surface expression is elevated upon viral infection. Engagement of SLAMF4 (CD244) by a natural ligand, CD48, leads to cell-mediated cytotoxicity and IFN-γ secretion by NK cells via the adaptor protein, SAP. While CD48-expressing tumors were efficiently eliminated by NK cells in SLAMF4-deficient mice, indicating that the CD48/SLAMF4 interaction plays an inhibitory role on NK cells in a context-dependent manner. In mice lacking expression of self-MHC-binding inhibitory receptors, ligation of CD48 and SLAMF4 inhibits NK cell activation, indicating that CD48/SLAMF4 contributes to NK-cell self-tolerance in a MHC-I-independent manner. Differential expression of CD48 in distinct subsets of human dendritic cells affects NK cell activation. In addition, CD48 interacts with SLAMF4 and functions as a costimulatory molecule, playing a critical role in cell proliferation and MHC-restricted cytotoxicity in CD8+ T cells. CD48 on the surface of marginal zone B cells activates NK cells and stimulates Th2 cytokine IL-13 production.
Product DetailsProduct Details
- Source
- Human CD48, amino acid Gln27-Ser220 (Accession # P09326.2), with a linker (GSSR) and a C-terminal human IgG (Pro100-Lys330), was expressed in 293E cells.
- Molecular Mass
- The 429 amino acid recombinant protein has a predicted molecular mass of approximately 48.68 kD. The DTT-reduced and non-reduced protein migrates at approximately 70 and 140 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When recombinant human CD48-Fc Chimera is immobilized at 5 µg/mL, biotinylated human SLAMF4-Fc Chimera binds with EC50 of 0.75 - 3 µg/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Widely expressed on hematopoietic cells except granulocytes, platelets, and erythrocytes. Increased on T and B cells after activation.
- Function
- Contributes to T cell antigen recognition, plays an inhibitory and activating role on NK cells
- Interaction
- NK cells, subsets of T cells; Lck, Fyn interact with cytoplasmic domain
- Ligand/Receptor
- SLAMF4 (CD244)
- Bioactivity
- Measured by its ability to bind human SLAMF4
- Cell Sources
- 293E cells
- Antigen References
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- Veillette A. 2004. J. Exp. Med. 199:1175.
- Fisher RC and Thorley-Lawson DA. 1991. Mol. Cell. Biol. 11:1614.
- Yokoyama S, et al. 1991. J. Immunol. 146:2192.
- Lee KM, et al. 2004. J. Exp. Med. 199:1245.
- Assarsson E, et al. 2005. J. Immunol. 175:2045.
- Korinek V, et al. 1991. Immunogenetics. 33:108.
- Knapp W, et al. 1989. Immunol. Today. 10:253-8.
- Schlossman S, et al. 1995. Oxford University Press
- McNerney ME, et al. 2005. Blood. 106:1337.
- Gao N, et al. 2006. J. Immunol. 176:2758.
- Morandi B, et al. 2005. J. Immunol. 175:3690.
- Messmer B, et al. 2006. J. Immunol. 176:4646.
- Lee KM, et al. 2003. J. Immunol. 170:4881.
- Kato K, et al. 1992. J. Exp. Med. 176:1241.
- Lee KM, et al. 2006. Blood. 107:3181.
- Gene ID
- 962 View all products for this Gene ID
- UniProt
- View information about CD48 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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