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- Regulatory Status
- RUO
- Other Names
- Hepatocyte growth factor, Hepatopoeitin-A, Hepatopoietin A, HGF, Lung-Fibroblast-derived Mitogen, HPTA, scatter factor, SF, fibroblast-derived tumor cytotoxic factor
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human HGF induces proliferation of mIMCD-3 cells in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.5 ng/mL. -
Stability Testing for recombinant human HGF. Recombinant human HGF was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to induce the proliferation of mIMCD-3 cells in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.5 ng/mL.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 584014 | 25 µg | ¥100,500 | |
| 584016 | 100 µg | ¥152,240 |
Hepatocyte growth factor (HGF) was initially purified from the plasma of a patient with fulminant hepatic failure. HGF is a dimeric molecule composed of the α-subunit and β-subunit, linked by a disulfide bond. The two chains are derived from a precursor encoded by the same open reading frame. The C-terminus of the α-subunit is followed directly by the N-terminus of the β-subunit. The pro-HGF possesses 728 amino acids and the subunits are formed by proteolytic cleavage. Proteolytic activation of the precursor form in the extracellular milieu is performed by HGF activator (HGFA). HGFA is a factor XII-like serine proteinase that is not inhibited by serum proteinase inhibitors. Two inhibitors have been described for HGFA: HGFA inhibitor type 1 and type 2 (HAI-1 and HAI-2). Both inhibitors have two well-defined Kunitz inhibitor domains and a presumed transmembrane domain. HGF protects the epithelium, neurons and cardiomyocytes during organ diseases; this protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules, such as Bcl-xL. In addition, HGF prohibits Fas-mediated apoptosis signals via sequestration of Fas and Met receptor on cell surfaces. HGF also elicits the regression of fibrosis in numerous organs, such as scleroderma, cardiomyopathy, vocal scarring, and peritoneal fibrosis. Human and murine HGFs are cross-reactive.
Product DetailsProduct Details
- Source
- Recombinant human HGF, amino acid Gln32-Ser728 (Accession No. P14210-1) was expressed in CHO cells. The C-terminal includes 6His.
- Molecular Mass
- The 707 amino acid recombinant protein has a predicted molecular mass of approximately 80.74 kD. The DTT-reduced proteins migrate at approximately 80 – 115 kD (alpha and beta chain), 55 – 65 kD (alpha chain), and 25 – 35 kD (beta chain) by SDS-PAGE. The N-terminal amino acid is Gln.
- Purity
- ≥ 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human HGF induces the proliferation of mIMCD-3 cells in a dose-dependent manner. The ED50 for this effect is 0.5 – 3.5 ng/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked heterodimer
- Distribution
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Platelets, mesenchymal cells, liver sinusoidal endothelial cells, macrophages
- Function
- A potent mitogen for mature parenchymal hepatocyte cells, hepatotrophic factor, and a growth factor for a broad spectrum of tissues and cell types. It acts as an activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization
- Interaction
- Hepathocytes, epithelial cell
- Ligand/Receptor
- MET/HGF receptor
- Bioactivity
- Measured by its ability to induce proliferation of mouse IMCD-3 cells
- Cell Type
- Endothelial cells, Macrophages, Mesenchymal cells, Platelets
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immuno-Oncology, Immunology
- Molecular Family
- Soluble Receptors
- Antigen References
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- Nakamura T, et al. 1989. Nature. 342:440-3.
- Gheradi E, et al. 2003. Proc Natl Acad Sci USA. 100:12039.
- Urbanek K, et al. 2005. Circulation. 97:663-73.
- Montesano R, et al. 2004. Cell. 67:901.
- Saelman EUM, et al. 1995. J Cell Sci. 108:351.
- Ko KR, et al. 2018. Sci Rep. 8:8316.
- D'Angelo F, et al. 2013. Clin Exp Immunol. 174:60-72.
- Gene ID
- 3082 View all products for this Gene ID
- UniProt
- View information about HGF on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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