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Recombinant Human IFN-γ (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF),
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Product Citations
publications
Cat # Size Price Save
570202 10 µg ¥16,520
570204 25 µg ¥29,260
570206 100 µg ¥45,580
570208 500 µg ¥139,900
Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Source
Human IFN-γ, amino acids Gln24-Gln166 (Accession # NM_000619) was expressed in E. coli.
Molecular Mass
The 144 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,907 Da. The DTT-reduced protein and the non-reduced protein migrate at approximately 17 kDa by SDS-PAGE.
Purity
Purity is >95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 10mM Sodium succinate, 4% Mannitol, pH 5.0.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human IFN-γ induced cytotoxic effect on HT-29 cells in a dose-dependent manner. The ED50 is 1 – 6 ng/mL.
Application

Bioassay
Recommended Usage

Use when high specific biological activity is required.

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References

(PubMed link indicates BioLegend citation)
  1. Meissner TB, et al. 2012. J. Immunol. 188:4951. PubMed
  2. Meissner TB, et al. 2010. Proc. Natl. Acad. Sci. USA 107:13794. PubMed
Product Citations
  1. Jiang Z, et al. 2021. J Clin Invest. 131: . PubMed
  2. Zhang Z, et al. 2022. Cancer Gene Ther. 29:722. PubMed
  3. Mavrikaki M, et al. 2022. Nat Aging. 2:1130. PubMed
  4. Arnold KA, et al. 2023. Int J Mol Sci. 24:. PubMed
  5. Nguyen DJM, et al. 2020. Mol Cancer Res. 0.822916667. PubMed
  6. Gilad Y, et al. 2019. Sci Rep. 9:15099. PubMed
  7. Tsai M, et al. 2022. J Biol Chem. :102698. PubMed
  8. Zhang C, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  9. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  10. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  11. Gruber C, et al. 2020. J Exp Med. 217:00:00. PubMed
  12. Rajan SS, et al. 2020. Oncogene. 39:2103. PubMed
  13. Zhang H, et al. 2020. Front Cell Dev Biol. 8:205. PubMed
  14. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  15. Nenasheva T, et al. 2020. Front Immunol. 1.163888889. PubMed
  16. Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed
  17. Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed
  18. Rafieerad A, et al. 2021. Adv Funct Mater. 31:2106786. PubMed
  19. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  20. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  21. Lin H, et al. 2020. Small. 16:e2002194. PubMed
  22. Yamaguchi Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  23. Brasil da Costa FH, et al. 2020. PLoS One. 15:e0230354. PubMed
  24. Sharma V, et al. 2021. ACR Open Rheumatol. 3:277. PubMed
  25. Wrenn ED, et al. 2020. Cell. 183:395. PubMed
  26. Miyamoto T, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  27. Shikama Y, et al. 2022. Inflammation. . PubMed
  28. Guo Q, et al. 2022. Blood Adv. 6:5668. PubMed
  29. Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
  30. Chen B, et al. 2021. Cell. 184:6262. PubMed
  31. Stebbing J, et al. 2021. Sci Adv. 7:00. PubMed
  32. Hogg SJ, et al. 2022. Clin Epigenetics. 14:96. PubMed

Antigen Details

Structure
Cytokine; dimer
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 9    Revision Date: 07/13/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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