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- Regulatory Status
- RUO
- Other Names
- OFC7, PRR, PRR1, PVRR, PVRR1, SK-12, HIgRPVRR, MGC142031, Nectin1, Nectin-1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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When recombinant human Nectin-1 is immobilized at 2 µg/mL, biotinylated human Nectin-3 binds in a dose-dependent manner. HRP Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 1.7 – 12 ng/mL. -
Stability Testing for Recombinant Human Nectin-1. Recombinant human Nectin-1 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested in a binding assay with the immobilized Recombinant Human Nectin-1 at 2 µg/mL and different concentrations of recombinant human Nectin-3 that binds in a dose-dependent manner. The ED50 for this effect is 1.7 – 12 ng/mL.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 565404 | 25 µg | ¥58,710 | |
| 565406 | 100 µg | ¥120,400 |
Nectin-1, also known as poliovirus receptor related 1 protein (PRR1) and CD111, is a type I transmembrane glycoprotein with three extracellular immunoglobulin domains, a single transmembrane helix, and a cytoplasmic tail. Nectin-1 is part of the immunoglobulin (Ig) gene superfamily and a member of the Nectin family: Nectin-1, Nectin-2, Nectin-3, and Nectin-4. Nectin-1 plays a key role in arbitrating cellular entry of the herpes simplex virus by interacting with the viral glycoprotein D (gD). Nectin-1 is broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells. Nectin-1 localizes in various junctions such as the chemical synapse of neurons and the Adherens Junctions (AJ) of epithelial tissue. Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers together with Nectin-3 or Nectin-4; Nectin-1 also binds to afadin, a multi-domain scaffold protein.
Product DetailsProduct Details
- Source
- Recombinant human Nectin-1, amino acid Gln31-Thr334 (Accession No. Q15223) was expressed in CHO cells.
- Molecular Mass
- The 317 amino acid recombinant protein has a predicted molecular mass of approximately 35.5 kD. The DTT-reduced and non-reduced proteins migrate at approximately 50-65 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.4
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized recombinant human Nectin-1 binds recombinant human Nectin-3 in a dose-dependent manner. The ED50 for this effect is 1.7 – 12 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Transmembrane glycoprotein
- Distribution
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Myelomonocytic cells, megakaryocytes, CD34-positive stem cells, endothelial cells, epithelial cells, and neuronal cells
- Function
- Adhesion, receptor for Herpes simplex Virus 1 (HSV-1) and HSV-2
- Interaction
- Endothelial cells, epithelial cells, hematopoietic stem and progenitors, megakaryocytes
- Ligand/Receptor
- Nectin-1, -2, -3, and -4, CD155, HSV-1 and HSV-2
- Bioactivity
- Measured by its ability to bind recombinant human Nectin-3
- Cell Type
- Endothelial cells, Epithelial cells, Megakaryocytes, Neurons
- Biology Area
- Immuno-Oncology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, Innate Immune Signaling
- Antigen References
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- Cocchi F, et al. 1999. Proc Natl Acad Sci USA. 95:15700.
- Takahashi K, et al. 1999. J Cell Biol. 145:8127-37.
- Togashi H, et al. 2006. J Cell Biol. 174:141-51.
- Tampakis A, et al. 2019. Oncology. 96:318-325.
- Takai Y, et al. 2008. Nat Rev Mol Cell Biol. 9:603-15.
- Suzuki K, et al. 2000. Nat Genet. 25:427-30.
- Gene ID
- 5818 View all products for this Gene ID
- UniProt
- View information about Nectin-1 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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