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- Regulatory Status
- RUO
- Other Names
- TGFRII, TGFR2, TGFR-2, TβRII, TGFbeta-RII, TGF-Beta Receptor Type II, TGF-Beta Receptor Type-2
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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When recombinant human TGF-β1 is immobilized at 2 µg/mL, recombinant human TGFBR2-Fc Chimera binds in a dose-dependent manner. Direct-Blot™ HRP anti-human IgG1 Fc (Cat. No. 410603) was used to detect the binding. The ED50 for this effect is 2 – 10 ng/mL. -
Stability Testing for Recombinant Human TGFBR2-Fc Chimera. Recombinant Human TGFBR2-Fc Chimera was aliquoted in 10mM Na2HPO4, 0.3M NaCl, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested in a binding assay with immobilized recombinant human TGF-β1 at 2 µg/mL and different concentration of human TGFBR2-Fc that binds in a dose-dependent manner. The ED50 for this effect is 2 – 10 ng/mL.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 794304 | 25 µg | ¥43,390 | |
| 794306 | 100 µg | ¥105,080 |
TGF-β1 and its receptors play an important role in normal development and homeostasis. TGF-β ligands (TGF-β1, TGF-β2, TGF-β3) bind to three receptor isoforms TGFBR1, TGFBR2, and TGFBR3. TGFBR1 and 2 are serine/threonine and tyrosine kinases; TGFBR3 does not possess kinase activity. The TGFBR1 and TGFBR2 have similar structure characterized by a cysteine rich extracellular domain, a transmembrane helix, and the intracellular serine-threonine kinase domain. The TGFBR2 binds with high affinity to the ligands, and this binding promotes the recruitment and transphosphorylation of TGFBR1. TGFBR2 is also present in soluble forms named TβRII-SE splice variant. This variant binds to the three TGFβs with high affinity. TGFBRII-SE has been used in a model of liver fibrosis, and overexpression of TGFBRII-SE-Fc decreases liver damage and reduces liver fibrosis. TGF-β1 and TGFBR2 play a key role in suppressive and inflammatory immune responses. TGF-β controls thymic T-cell selection and homeostasis of naïve T-cell. TGF-β1 promotes peripheral Treg, Th9, Th17, and Tfh cell generation. In addition, TGF-β1 suppresses CTL, Th1, and Th2 cells.
Product DetailsProduct Details
- Source
- Recombinant human TGFBR2-Fc Chimera, amino acid sequence containing Thr23-Asp159 (Accession No. P37173), was expressed in 293E cells. The carboxy-terminus contains SR-IEGRMD-hIgG1 (Pro100-Lys330)-Fc tag.
- Molecular Mass
- The 376 amino acid recombinant protein has a predicted molecular mass of approximately 42.4 kD. The DTT-reduced and non-reduced proteins migrate at approximately 60 kD and 100 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Thr.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 10 mM Na2HPO4, 0.3 M NaCl, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized recombinant human TGF-β1 (Cat. No. 781802) binds to human TGFBR2-Fc in a dose dependent manner. The ED50 for this effect is 2 – 10 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer
- Distribution
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Ubiquitous expression, endothelial cells, adipocytes, alveolar cells, thymic epithelial cells, fibroblasts, T cells, monocytes, B cells, vascular smooth muscle cells
- Function
- Extracellular matrix deposition, adhesion, cellular proliferation, epithelial to mesenchymal transition, wound healing, tumorigenesis, and immune system role
- Interaction
- Interacts with TGFβ-ligands and TGFBR1
- Ligand/Receptor
- TGF-β1, TGF-β2, and TGF-β3
- Bioactivity
- Measured by its ability to bind recombinant human TGF-β1
- Cell Type
- Tfh, Th1, Th17, Th2, Tregs
- Biology Area
- Adaptive Immunity, Cancer Biomarkers, Cell Proliferation and Viability
- Molecular Family
- Fc Receptors, Soluble Receptors
- Antigen References
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- Vander Ark A, et al. 2018. Cell Signaling. 52:112.
- Gropee J, et al. 2008. Moll Cell. 29:157.
- Bertolio MS, et al. 2021. Front Cell Dev Biol. 9: 90397.
- Marie JC, et al. 2006. Immunity 25:441.
- Sanjabi S, et al.. 2017. Cold Spring Harb Perspective Biol. 9:a022236.
- Ghasempour G, et al. 2022. Mol Biol Rep. 49:2869.
- Gene ID
- 7048 View all products for this Gene ID
- UniProt
- View information about TGFBR2 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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