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- Regulatory Status
- RUO
- Other Names
- Neural Cell Adhesion Molecule 1, NCAM, CD56, NCAM-1, CD56 antigen
- Ave. Rating
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- Product Citations
- publications
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Immobilized mouse CD56 supports the adhesion of Neuro-2A cells in a dose-dependent manner. The ED50 for this effect is of 0.3 – 1.8 µg/mL. -
Stability Testing for Recombinant Mouse CD56. Recombinant mouse CD56 was aliquoted in PBS at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to support the adhesion of Neuro-2A cells in a dose-dependent manner. The ED50 for this effect is 0.3 – 1.8 µg/mL.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 565204 | 25 µg | ¥62,690 | |
| 565206 | 100 µg | ¥124,380 |
CD56, also known as neural cell adhesion molecule 1 (NCAM-1), belongs to the cell adhesion molecules (CAMs) of the immunoglobulin superfamily (IgSF-CAMs). The structure of NCAM consists of five immunoglobulin-like (Ig) domains followed by two fibronectin type III (FNIII) domains, and plays a crucial role in neuronal development and synaptic plasticity. The Ig domains of CD56 are involved in homophilic binding, promoting cell-cell adhesion and helping in synaptic formation and maintenance. The FNIII domains are involved in signaling to induce neurite outgrowth. CD56 can also induce neuronal differentiation by activating several signaling molecules. Alterations in CD56 expression and CD56-mediated signaling have been associated with cognitive deficits, such as schizophrenia and Alzheimer's disease. In addition, studies have shown a positive correlation between CD56 and Hereditary Motor Sensory Neuropathy (CMT). CD56 is a significant protein involved in various neural processes, and its dysregulation has implications in several neurological conditions.
Product DetailsProduct Details
- Source
- Mouse CD56, amino acid Leu20-Thr711 (Accession: P13595.3), with a C-terminal 6 His, was expressed in 293E.
- Molecular Mass
- The 698 amino acid recombinant protein has a predicted molecular mass of approximately 77.43 kD. The DTT-reduced and non-reduced protein shall migrate at approximately 75 -115 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- Purity
- > 95%, as determined by Coomassie-stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Immobilized recombinant mouse CD56 supports the adhesion of Neuro-2A cells in a dose-dependent manner with ED50 range of 0.3 – 1.8 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer
- Distribution
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NK cells, T cells, neurons
- Function
- Induce neurite outgrowth, neuronal differentiation, and helps in the formation and stabilization of synapses between neurons
- Interaction
- Fibroblast growth factor receptors (FGFRs)
- Ligand/Receptor
- MDK (Midkine receptor)
- Bioactivity
- Mouse CD56 supports the adhesion of N2A cells in a dose-dependent manner.
- Cell Type
- B cells, Leukemia, Mesenchymal Stem Cells, Neurons, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Kim W, et al. 2021. J Biol Chem. 296:100372.
- Ditlevsen DK, et al. 2008. J Neurosci Res. 86:727-43.
- Sytnyk V, et al. 2017. Trends Neurosci. 40:295-308.
- Weledji EP and Assob JC. 2014. Ann Med Surg. 3:77-81.
- Berridge MJ. 2013. Prion. 7:2-13.
- Chu C, et al. 2018. J Cell Biol. 43:1714.
- Muramatsu H, et al. 2000. Biochem Biophys Res Commun. 270:936-41.
- Kos FJ and Chin CS. 2002. Immunol Cell Biol. 80:364-9.
- Jennings MJ, et al. 2022. Brain. 145:3999-4015.
- Gene ID
- 17967 View all products for this Gene ID
- UniProt
- View information about CD56/NCAM-1 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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