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- Regulatory Status
- RUO
- Other Names
- GPC1, GPC-1
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- Product Citations
- publications
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When mouse Glypican-1 is immobilized at 2 µg/mL, human FGF-basic binds with EC50 of 3 - 12 ng/mL in a functional ELISA.
Glypicans are heparin sulfate proteoglycans that are bound to the outer cell surface membrane by a glycosyl-phosphatidylinositol (GPI) linkage. There are six known mammalian Glypicans (GPC1 to GPC6) and they can be released from the cell surface by a lipase called Notum. The main function of membrane bound glypicans is to regulate the signaling of Wnts, Hedgehogs, fibroblast growth factors, and bone morphogenetic proteins. Glypican-1 has been associated with the tumorigenic process, mostly by affecting growth factor signaling and cell proliferation. Glypican-1, also known as GPC1, shows increased expression in human gliomas, acts by enhancing fibroblast growth factor (FGF) basic signaling, and mitogenesis. GPC1 is over-produced in pancreatic and breast cancer cells and has been shown to be a marker of cancer-derived exosomes in these cancers. GPC1 is also shown to associate with fibrillar APP Aβ peptides in lipid rafts of Alzheimer disease brains. GPC1 is synthesized as a 558 amino acid preproprecursor that contains a 23 amino acid signal peptide, a 507 amino acid mature segment, and a 28 amino acid C-terminal propeptide. There are two potential N-linked and three potential O-linked sites for glycosylation and potentially three heparan sulfate modifications on GPC1. GPC1 is expressed on neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells. Mature mouse GPC1 shares a 91% amino acid identity with mature human GPC1.
Product DetailsProduct Details
- Source
- Mouse Glypican-1, amino acids Asp24-Ser530 (Accession# Q9QZF2) with C-terminal 6-His, was expressed in 293E cells.
- Molecular Mass
- The 520 amino acid recombinant protein has a predicted molecular mass of approximately 57.5 kD. The protein migrates at approximately 65 kD in DTT-reducing conditions and at approximately 60 kD in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Asp.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When mouse Glypican-1 is immobilized at 2 µg/mL, human FGF-basic binds with EC50 of 3 - 12 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Distribution
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Glypican-1 is expressed on neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells.
- Function
- The main function is to regulate the signaling of fibroblast growth factors.
- Interaction
- Fibroblast growth factors, extracellular matrix, and APP Abeta peptides.
- Ligand/Receptor
- Fibroblast growth factors and laminin.
- Bioactivity
- Measured by the ability of immobilized mouse Glypican-1 to bind human FGF-basic in a functional ELISA.
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Antigen References
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1. Kleeff J, et al. 1998. J. Clin. Invest. 102:1662-73.
2. Gengrinovitch S, et al. 1999. J. Biol. Chem. 274:10816-22.
3. Matsuda K, et al. 2001. Cancer Res. 14:5562-9.
4. Qiao D, et al. 2003. 278:16045-53.
5. Watanabe N, et al. 2004. FASEB J. 9:1013-5.
6. Bishop JR, et al. 2007. Nature 446:1030-7.
7. Aikawa T, et al. 2008. J. Clin. Invest. 118:89-99.
8. Melo SA, et al. 2015. Nature 523:177-182. - Gene ID
- 14733 View all products for this Gene ID
- UniProt
- View information about Glypican-1 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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