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- Regulatory Status
- RUO
- Other Names
- Interleukin-22, IL-TIF
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- Product Citations
- publications
IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.
Product DetailsProduct Details
- Source
- Mouse IL-22, amino acids Leu34-Val179 (Accessio # NM_016971) was expressed in E. coli.
- Molecular Mass
- The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 16.8 kD. The DTT-reduced protein migrates at approximately 15 kD and the non-reduced protein migrates at approximately 13 kD by SDS-PAGE.
- Purity
- Purity is >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
- Endotoxin Level
- Endotoxin level is <0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
- Application
-
ELISA - Quality tested
- Recommended Usage
-
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/ml to 3.9 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This IL-22 protein is useful as a standard in a mouse IL-22 sandwich ELISA, using unlabeled poly5164 antibody (Cat. No. 516401) for capture and biotinylated poly5164 antibody (Cat. No. 516407) for detection.
Antigen Details
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Dumoutier L, et al. 2000. J. Immun. 164:1814.
2. Xie MH, et al. 2000. J. Biol. Chem. 275:31335.
3. Jones BC, et al. 2008. Structure.
4. Kotenko SV, et al. 2001. J. Immun. 166:7096.
5. Chang C, et al. 2006. Cell Research 16:902. - Gene ID
- 50929 View all products for this Gene ID
- UniProt
- View information about IL-22 on UniProt.org
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