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- Regulatory Status
- RUO
- Other Names
- Junctional adhesion molecule-like, Adhesion molecule interacts with CXADR antigen 1, AMICA
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Mouse JAML supports the adhesion of HeLa cells in a dose dependent manner. ED50 for this effect is 0.5 - 2.0 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells. -
Stability testing for Mouse JAML. Mouse JAML was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested for their ability to support the adhesion of HELA cells.
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Junctional adhesion molecule-like (JAML), also known as AMICA, is a type I transmembrane glycoprotein that belongs to junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily. JAM family members contribute to intercellular connections within epithelial and endothelial cell layers by facilitating tight junction assembly. They also orchestrate recruitment of leukocytes to sites of inflammation and wound repair. JAML expression is restricted to the hematopoietic tissues, and is upregulated during the differentiation of myeloid leukemia cells. JAML controls leukocyte migration and activation by interacting with CXADR. JAML and CXADR interaction induces the activation of gamma-delta T-cells through PI3-kinase and MAP kinases which leads to proliferation of T cells and production of cytokines and growth factors. JAML is implicated in tissue homeostasis and repair. JAML also mediates adhesion of monocytes to endothelial cells, and neutrophil migration across epithelial cell monolayers.
Product DetailsProduct Details
- Source
- Mouse JAML, amino acids Leu23-Leu281 (Accession # Q80UL9) with a C-terminal human IgG Fc (Pro100-Lys330) was expressed in 293E cells.
- Molecular Mass
- The 500 amino acid recombinant protein has a predicted molecular mass of approximately 56.5 kDa. The DTT-reduced protein migrates at approximately 70 kDa and non-reduced protein migrates at approximately 140 kDa by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.5 - 2.0 μg/mL as measured by the ability of immobilized protein to support the adhesion of HeLa cells. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
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Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. Preferentially expressed by mature leukocytes.
- Function
- Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR.
- Interaction
- Epithelial and endothelial cells, leukocytes.
- Ligand/Receptor
- CXADR
- Bioactivity
- Support adhesion of HeLa cells.
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, Soluble Receptors
- Gene ID
- 270152 View all products for this Gene ID
- UniProt
- View information about JAML on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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