United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Regulatory Status
- RUO
- Other Names
- interleukin-17F, interleukin 17F, IL17F, IL-17F, cytokine ML-1, ML1, ML-1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant rat IL-17F induces the production of mIL-6 proteins in fetal mouse skin fibroblast cells in a dose-dependent manner. The ED50 for this effect is 80 – 800 ng/mL. -
Stability testing for recombinant rat IL-17F. Recombinant rat IL-17F was aliquoted in PBS at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to induce production of mIL-6 proteins in fetal mouse skin fibroblast cells in a dose-dependent manner. The ED50 for this effect is 80 – 800 ng/mL.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 799704 | 25 µg | ¥69,650 | |
| 799706 | 100 µg | ¥153,230 |
Interleukin 17F (IL-17F) belongs to the IL-17 cytokine family which includes IL-17A, B, C, D, and E (also called IL-25). IL-17F shares the strongest homology to IL-17A. They share 50% amino acid sequence homology. IL-17-IL-17F heterodimer was found to be expressed in Th17 cells together with IL-17 and IL-17F homodimers. IL-17 and IL-17F gene promoters share the same pattern of chromatin remodeling in differentiated Th17 cells. T cell populations in vitro and in vivo have shown different ratios of IL-17 and IL-17F expression. IL-17, IL-17F regulates proinflammatory gene expression in vitro. Studies in vivo using IL17a(-/- ), IL17f(-/-), and IL17a(-/-)IL17f(-/-) mice showed that IL-17F played only marginal roles, if at all, in the development of delayed-type and contact hypersensitivities, autoimmune encephalomyelitis, collagen-induced arthritis, and arthritis in mice deficient in IL-1 receptor antagonist. In contrast, both IL-17F and IL-17A were involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium. Additional studies in vivo have suggested a role for IL-17F in the induction of neutrophilia in the lungs and in the exacerbation of Ag-induced pulmonary inflammation.
Product DetailsProduct Details
- Source
- Recombinant rat IL-17F, amino acid Ala28-Ala161 (Accession No. Q5BJ95) was expressed in E.coli
- Molecular Mass
- The 135 amino acid recombinant protein has a predicted molecular mass of 15.0 kD and contains methionine as N-terminal. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 16 kD by SDS-PAGE. The non-reduced protein migrates as a homodimer, at approximately 29 kD by SDS-PAGE
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant rat IL-17F induces IL-6 in fetal mouse skin fibroblasts in a dose-dependent manner. The ED50 for this effect is 80 - 800 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Cytokine
- Distribution
-
T cells, Th17 cells, memory CD4 T cells, innate immune cells, and epithelial cells
- Function
- IL-17F induces proliferation, differentiation, cell survival, and apoptosis. In addition, IL-17F induces IL-6 and KC (CXCL1) production in mouse fibroblast and macrophages, stimulates granulopoiesis in mice, and negative regulation of IL-17 production in T cells. Pathophysiological processes, viral infections and autoimmune diseases.
- Interaction
- IL-17F might act primarily on parenchymal cells such as fibroblasts, epithelial cells, and endothelial cells.
- Ligand/Receptor
- IL-17RC, IL-17F can signal via the IL-17RA; however, with lower affinity than IL17A.
- Bioactivity
- IL-17F induces IL-6 and KC (CXCL1) production in mouse fibroblast and macrophages. IL-17F/IL-17R interaction stimulates granulopoiesis in mice. IL-17F homodimer via IL-17RA is a negative regulator of IL-17 production in T cells.
- Cell Type
- Epithelial cells, T cells, Th17
- Biology Area
- Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors, Cytokines/Chemokines, Innate Immune Signaling
- Antigen References
-
- Starnes T, et al. 2001 J Immunol. 167:4137-40.
- Kawaguchi M, et al. 2001 J Immunol. 167:4430-5.
- Kolls JK and Linden A. 2004. Immunity. 21:467-76.
- Oda N, et al. 2005. Am J Respir Cell Mol Biol. 171:12.
- Hizawa N, et al. 2006. Clin Experimental Allergy. 36:1109-14.
- Weaver CT, et al. 2007. Annu Rev Immunol. 25:801-52.
- Chang, SH and C Gong. 2007. Cell Res. 17:435-40.
- Seiderer J, et al. 2008. Inflamm Bowel Dis. 14:437-45.
- Gene ID
- 301291 View all products for this Gene ID
- UniProt
- View information about IL-17F on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
-
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
-
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
-
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
-
Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
Follow Us