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- Regulatory Status
- RUO
- Other Names
- P40, MEA (mast cell growth enhancing activity), MCGF (Mast cellgrowth factor), TCGF-3 (T cell growth factor-3)
- Ave. Rating
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- Product Citations
- publications
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Rat IL-9 induces proliferation of MC/9 mouse mast cells in a dose dose-dependent manner. The ED50 for this effect is 80 - 800 ng/mL. -
Stability Testing for Recombinant Rat IL-9. Recombinant rat IL-9 was aliquoted in PBS and pH 7.2. at 0.2 mg/mL. One aliquot was kept at 4°C (control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to induce the proliferation of MC/9 mouse mast cells in a dose-dependent manner. The ED50 for this effect is 80 – 800 ng/mL.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 569804 | 25 µg | ¥117,410 | |
| 569806 | 100 µg | ¥220,890 |
Interleukin-9 (IL-9) regulates variety of hematopoietic cells, stimulates cell proliferation, and prevents apoptosis. Mature rat IL-9 shows 58% and 74% amino acid sequence homology with human and mouse IL-9, respectively. The IL-9 receptor consists of the cytokine-specific IL-9 receptor α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4, IL-7, IL-15 and IL-21. IL-9 is expressed by activated Th9, Th17, Treg, and mast cells and appears to play important roles in the development and function of subsets within the CD4+ T cell lineage. IL-9 also inhibits immune responses by enhancing the suppressive properties of Tregs and by recruiting immune suppressive mast cells to sites of inflammation. Genetic studies on a mouse model of asthma demonstrated that IL-9 plays a key role in the pathogenesis of bronchial hyperresponsiveness correlating with the observed increased expression of IL-9 and IL-9R in lungs of asthmatic patients. In addition, IL-9 promotes mast cell mediated intestinal permeability, development of food allergies and atopic dermatitis.
Product DetailsProduct Details
- Source
- Recombinant rat IL-9, amino acid Gln19-Ala144 (Accession No. D4A819) was expressed in 293E.
- Molecular Mass
- The 126 amino acid recombinant protein has a predicted molecular mass of approximately 14.1 kD. The DTT-reduced and non-reduced protein migrate at approximately 27 - 35 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 80 – 800 ng/mL, as determined by a dose-dependent stimulation in a proliferation assay with MC/9 cells. Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation.
- Application
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Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Th2 cells, mast cells, activated Th9, Th17, and Treg cells
- Function
- Autocrine growth and activation factor for Th17, Tregs, and mast cells. IL-1, histamine, and LPS induce the expression of IL-9 in mast cells. IL-9 is induced synergistically by IL-2, IL-4, and TGFβ and inhibited by IFNγ in naÏve CD4 T cells
- Interaction
- T helper cells, Th9, Th17, Tregs, human airway smooth muscle cells
- Ligand/Receptor
- IL-9R complex (IL-9Rα and γ chain)
- Bioactivity
- rat IL-9 induces proliferation of MC/9 cells
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
- Goswami R & Kaplan MH. 2011. J Immunol. 186:3283-8.
- Kaiko GE & Foster PS. 2011. Curr Opin Allergy Clin Immunol. 11:39-45.
- Townsend JM, et al. 2000. Immunity. 13:573-83.
- Mock BA, et al. 1990. Immunogenetics. 31:265-70.
- Tan C, et al. 2010. J Immunol. 185:6795-801.
- Jäger A, et al. 2009. J Immunol. 183:7169-77.
- Sismanopoulos N, et al. 2012. PLoS One. 7(3): e33271.
- Ye D, et al. 2020. Life Sci. 255:117844.
- Gene ID
- 116558 View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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