Spark Blue™ 574 anti-human CD107a (LAMP-1) (Flexi-Fluor™) Antibody, CD107a, H4A3

Clone: H4A3 (See other available formats)
Regulatory Status: RUO
Workshop: P PR-63; BP 473; P P008
Other Names: Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype: Mouse IgG1, κ
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Product Citations: publications

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Cat #	Size	Price	Save
285174	50 µg	¥86,680

Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8⁺ T cell and NK cell cytotoxicity.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human adult adherent peripheral blood cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting⁸, immunohistochemical staining², immunofluorescence^5,7, and immunoprecipitation⁵.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3662514 (BioLegend Cat. No. 285174)

Antigen Details

Structure: LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution: Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes
Function: Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor: Galaptin
Cell Type: Endothelial cells, Epithelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.
Gene ID: NA
UniProt: View information about CD107a on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD107a Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD107a (LAMP-1)	H4A3	FC
Purified anti-human CD107a (LAMP-1)	H4A3	FC,ICC,WB,IHC-P,IP,SB
FITC anti-human CD107a (LAMP-1)	H4A3	FC
PE anti-human CD107a (LAMP-1)	H4A3	FC
Alexa Fluor® 488 anti-human CD107a (LAMP-1)	H4A3	FC
Alexa Fluor® 647 anti-human CD107a (LAMP-1)	H4A3	FC
PerCP/Cyanine5.5 anti-human CD107a (LAMP-1)	H4A3	FC
APC anti-human CD107a (LAMP-1)	H4A3	FC
Pacific Blue™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 421™ anti-human CD107a (LAMP-1)	H4A3	FC
PE/Cyanine7 anti-human CD107a (LAMP-1)	H4A3	FC
APC/Cyanine7 anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 510™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 605™ anti-human CD107a (LAMP-1)	H4A3	FC
Purified anti-human CD107a (LAMP-1) (Maxpar® Ready)	H4A3	FC,CyTOF®
Brilliant Violet 650™ anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 711™ anti-human CD107a (LAMP-1)	H4A3	FC
PerCP anti-human CD107a (LAMP-1)	H4A3	FC
Brilliant Violet 785™ anti-human CD107a (LAMP-1)	H4A3	FC
PE/Dazzle™ 594 anti-human CD107a (LAMP-1)	H4A3	FC
TotalSeq™-A0155 anti-human CD107a (LAMP-1)	H4A3	PG
TotalSeq™-C0155 anti-human CD107a (LAMP-1)	H4A3	PG
TotalSeq™-B0155 anti-human CD107a (LAMP-1)	H4A3	PG
APC/Fire™ 750 anti-human CD107a (LAMP-1) Antibody	H4A3	FC
PE/Cyanine5 anti-human CD107a (LAMP-1)	H4A3	FC
Spark Blue™ 574 anti-human CD107a (LAMP-1) (Flexi-Fluor™)	H4A3	FC
Spark PLUS B550™ anti-human CD107a (LAMP-1) Antibody	H4A3	FC
Spark Red™ 718 anti-human CD107a (LAMP-1) Antibody	H4A3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.