Spark Blue™ 574 anti-human CD154 (Flexi-Fluor™) Antibody, CD154, 24-31

Clone: 24-31 (See other available formats)
Regulatory Status: RUO
Other Names: CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype: Mouse IgG1, κ
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Product Citations: publications

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Cat #	Size	Price	Save
285150	50 µg	¥86,680

Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy^1,3 and blocking of T cell-dependent B cell differentiation^1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3662490 (BioLegend Cat. No. 285150)

Antigen Details

Structure: TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.
Gene ID: NA
UniProt: View information about CD154 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD154 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD154	24-31	FC,IHC-F
FITC anti-human CD154	24-31	FC,IHC-F
PE anti-human CD154	24-31	FC
PE/Cyanine5 anti-human CD154	24-31	FC
APC anti-human CD154	24-31	FC
Biotin anti-human CD154	24-31	FC,IHC-F
Alexa Fluor® 488 anti-human CD154	24-31	FC,IHC-F
Alexa Fluor® 647 anti-human CD154	24-31	FC,IHC-F
Pacific Blue™ anti-human CD154	24-31	FC
Brilliant Violet 785™ anti-human CD154	24-31	FC
APC/Cyanine7 anti-human CD154	24-31	FC
Brilliant Violet 421™ anti-human CD154	24-31	FC,IHC-F
Brilliant Violet 605™ anti-human CD154	24-31	FC
Ultra-LEAF™ Purified anti-human CD154	24-31	FC,Block,IHC-F
Brilliant Violet 510™ anti-human CD154	24-31	FC,IHC-F
PE/Cyanine7 anti-human CD154	24-31	FC
PerCP/Cyanine5.5 anti-human CD154	24-31	FC
Brilliant Violet 711™ anti-human CD154	24-31	FC
Purified anti-human CD154 (Maxpar® Ready)	24-31	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD154	24-31	FC
TotalSeq™-A0032 anti-human CD154	24-31	PG
Alexa Fluor® 700 anti-human CD154	24-31	FC
APC/Fire™ 750 anti-human CD154	24-31	FC
TotalSeq™-C0032 anti-human CD154	24-31	PG
TotalSeq™-B0032 anti-human CD154	24-31	PG
TotalSeq™-D0032 anti-human CD154	24-31	PG
PE/Fire™ 640 anti-human CD154	24-31	FC
PE/Fire™ 810 anti-human CD154	24-31	FC
PerCP/Fire™ 780 anti-human CD154	24-31	FC
PE/Fire™ 700 anti-human CD154	24-31	FC
Spark Red™ 718 anti-human CD154 (Flexi-Fluor™)	24-31	FC
Brilliant Violet 650™ anti-human CD154	24-31	FC
Spark Blue™ 574 anti-human CD154 (Flexi-Fluor™)	24-31	FC
Spark Blue™ 550 anti-human CD154 (Flexi-Fluor™)	24-31	FC
PE/Cyanine7 anti-human CD154	24-31	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.