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Spark Blue™ 515 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin- Spark Blue™ 515, SAv- Spark Blue™ 515, streptavidin Spark Blue™ 515, SAV
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Product Citations
publications
Spark-Blue-515_Streptavidin_Antibody_102824
Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3 (clone UCHT1) (left) or biotinylated mouse IgG1, κ isotype control (right). Cells were then stained with anti-human CD4 (clone SK3) Alexa Fluor® 700 and SAV-Spark Blue™ 515.
  • Spark-Blue-515_Streptavidin_Antibody_102824
    Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3 (clone UCHT1) (left) or biotinylated mouse IgG1, κ isotype control (right). Cells were then stained with anti-human CD4 (clone SK3) Alexa Fluor® 700 and SAV-Spark Blue™ 515.
See Spark Blue™ 515 spectral data
Cat # Size Price Save
405389 25 µg ¥23,320
405390 100 µg ¥55,660
Description

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
Streptavidin is conjugated with Spark Blue™ 515 under optimal conditions.
Concentration
0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin Spark Blue™ 515 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark Blue™ 515 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 515 has a maximum excitation of 490 nm and a maximum emission of 515 nm.

Excitation Laser
Blue Laser (488 nm)

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 10/29/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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