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Spark NIR™ 685 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin- Spark NIR™, SAv- Spark NIR™ 685, streptavidin Spark NIR™ 685
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Product Citations
publications
Streptavidin_Spark-Violet-423_Antibody_FC_0060823
Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and biotinylated anti-human CD3 (left) or with biotinylated mouse IgG1, κ isotype control (right), followed with SAV-Spark NIR™ 685.
  • Streptavidin_Spark-Violet-423_Antibody_FC_0060823
    Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and biotinylated anti-human CD3 (left) or with biotinylated mouse IgG1, κ isotype control (right), followed with SAV-Spark NIR™ 685.
See Spark NIR™ 685 spectral data
Cat # Size Price Save
405371 25 µg ¥23,320
405372 100 µg ¥55,660
Description

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
Streptavidin is conjugated with Spark NIR™ 685 under optimal conditions.
Concentration
0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin Spark NIR™ 685 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark NIR™ 685 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.

Excitation Laser
Red Laser (633 nm)

Related FAQs

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Go To Top Version: 1    Revision Date: 05/26/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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