Spark PLUS B550™ anti-human CD38 Antibody, CD38, HIT2

Clone: HIT2 (See other available formats)
Regulatory Status: RUO
Workshop: III 155
Other Names: T10, ADP-ribosyl cyclase
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

HIT2_Spark-PLUS-B550_CD38_FC_090424 — Human peripheral blood lymphocytes were stained with anti-human CD19 (clone HIB19) APC and anti-human CD38 (clone HIT2) Spark PLUS B550™ (left) or stained with anti-human CD19 (clone HIB19) APC only (right).

Compare all formats See Spark PLUS B550™ spectral data

Cat #	Size	Price	Save
303563	25 tests	¥30,800
303564	100 tests	¥72,600

Description

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Horse, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark PLUS B550™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS B550™ has a maximum excitation of 516 nm and a maximum emission of 540 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶ and spatial biology (IBEX)^10,11.

Antigen Details

Structure: ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
Distribution: T cells, B cells, NK, myeloid, plasma, and dendritic cells
Function: Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
Ligand/Receptor: CD31, hyaluronic acid
Cell Type: B cells, Dendritic cells, NK cells, Plasma cells, T cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151.
2. Lund F, et al. 1995. Immunol. Today 16:469.
Gene ID: 952 View all products for this Gene ID
UniProt: View information about CD38 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Other Formats

View All CD38 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD38	HIT2	FC
FITC anti-human CD38	HIT2	FC
PE anti-human CD38	HIT2	FC
PE/Cyanine5 anti-human CD38	HIT2	FC
Purified anti-human CD38	HIT2	FC,CyTOF®,IHC-F
Alexa Fluor® 488 anti-human CD38	HIT2	FC
Alexa Fluor® 647 anti-human CD38	HIT2	FC
PE/Cyanine7 anti-human CD38	HIT2	FC
Biotin anti-human CD38	HIT2	FC
PerCP anti-human CD38	HIT2	FC
PerCP/Cyanine5.5 anti-human CD38	HIT2	FC
Alexa Fluor® 700 anti-human CD38	HIT2	FC,SB
Brilliant Violet 421™ anti-human CD38	HIT2	FC
Brilliant Violet 711™ anti-human CD38	HIT2	FC
Brilliant Violet 785™ anti-human CD38	HIT2	FC
Brilliant Violet 605™ anti-human CD38	HIT2	FC
APC/Cyanine7 anti-human CD38	HIT2	FC
Purified anti-human CD38 (Maxpar® Ready)	HIT2	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD38	HIT2	FC
PE anti-human CD38	HIT2	FC
Brilliant Violet 510™ anti-human CD38	HIT2	FC
FITC anti-human CD38	HIT2	FC
PE/Dazzle™ 594 anti-human CD38	HIT2	FC
TotalSeq™-A0389 anti-human CD38	HIT2	PG
TotalSeq™-C0389 anti-human CD38	HIT2	PG
APC/Fire™ 750 anti-human CD38	HIT2	FC
TotalSeq™-B0389 anti-human CD38	HIT2	PG
APC/Fire™ 810 anti-human CD38	HIT2	FC
Spark NIR™ 685 anti-human CD38 Antibody	HIT2	FC
TotalSeq™-D0389 anti-human CD38	HIT2	PG
PerCP/Cyanine5.5 anti-human CD38	HIT2	FC
APC anti-human CD38	HIT2	FC
APC/Fire™ 750 anti-human CD38	HIT2	FC
PE/Cyanine7 anti-human CD38	HIT2	FC
GMP PE anti-human CD38	HIT2	FC
GMP FITC anti-human CD38	HIT2	FC
Pacific Blue™ anti-human CD38	HIT2	FC
Pacific Blue™ anti-human CD38	HIT2	FC
GMP APC/Fire™ 750 anti-human CD38	HIT2	FC
GMP PE/Dazzle™ 594 anti-human CD38	HIT2	FC
GMP APC anti-human CD38	HIT2	FC
GMP PE/Cyanine7 anti-human CD38	HIT2	FC
Spark Blue™ 515 anti-human CD38	HIT2	FC
GMP Pacific Blue™ anti-human CD38	HIT2	FC
Spark Violet™ 423 anti-human CD38	HIT2	FC
Brilliant Violet 650™ anti-human CD38	HIT2	FC
Spark PLUS B550™ anti-human CD38	HIT2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/28/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.