- Clone
- UCHT2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III 518
- Other Names
- Leu-1, Ly-1, T1, Tp67
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Capuchin Monkey, Common Marmoset, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting2 and immunohistochemical staining of acetone-fixed frozen sections2,5.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
- Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC)
- Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437.
- Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed
- Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Antigen Details
- Structure
- Scavenger receptor superfamily, 67 kD
- Distribution
-
T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells
- Function
- Modulates TCR, BCR signaling, thymocyte maturation, T-B cell interaction
- Ligand/Receptor
- CD72
- Cell Type
- B cells, Dendritic cells, Leukemia, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kipps T. 1988. Adv. Immunol. 47:117.
2. Resnick D, et al. 1993. Trends Biochem. Sci. 19:5.
3. Wood GS, et al. 1992. Am. J. Pathol. 14:789. - Gene ID
- 921 View all products for this Gene ID
- UniProt
- View information about CD5 on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD5 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-human CD5 | UCHT2 | FC |
Biotin anti-human CD5 | UCHT2 | FC |
FITC anti-human CD5 | UCHT2 | FC |
PE anti-human CD5 | UCHT2 | FC |
Purified anti-human CD5 | UCHT2 | FC,IHC-F,WB |
Alexa Fluor® 647 anti-human CD5 | UCHT2 | FC |
PerCP anti-human CD5 | UCHT2 | FC |
PerCP/Cyanine5.5 anti-human CD5 | UCHT2 | FC |
PE/Cyanine7 anti-human CD5 | UCHT2 | FC |
Pacific Blue™ anti-human CD5 | UCHT2 | FC |
Brilliant Violet 421™ anti-human CD5 | UCHT2 | FC |
Purified anti-human CD5 (Maxpar® Ready) | UCHT2 | FC,CyTOF® |
APC/Cyanine7 anti-human CD5 | UCHT2 | FC |
Alexa Fluor® 700 anti-human CD5 | UCHT2 | FC |
PE/Dazzle™ 594 anti-human CD5 | UCHT2 | FC |
TotalSeq™-A0138 anti-human CD5 | UCHT2 | PG |
TotalSeq™-C0138 anti-human CD5 | UCHT2 | PG |
APC/Fire™ 750 anti-human CD5 | UCHT2 | FC |
Brilliant Violet 711™ anti-human CD5 | UCHT2 | FC |
TotalSeq™-B0138 anti-human CD5 Antibody | UCHT2 | PG |
TotalSeq™-D0138 anti-human CD5 | UCHT2 | PG |
Brilliant Violet 510™ anti-human CD5 | UCHT2 | FC |
Spark Red™ 718 anti-human CD5 (Flexi-Fluor™) | UCHT2 | FC |
Brilliant Violet 605™ anti-human CD5 | UCHT2 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UC... -
Biotin anti-human CD5
Human peripheral blood lymphocytes were stained with biotiny... -
FITC anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UC... -
PE anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
Purified anti-human CD5
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 647 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UC... -
PerCP anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UC... -
PerCP/Cyanine5.5 anti-human CD5
Human peripheral blood lymphocytes were stained with CD3 APC... -
PE/Cyanine7 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UC... -
Pacific Blue™ anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (UC... -
Brilliant Violet 421™ anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
Purified anti-human CD5 (Maxpar® Ready)
Human PBMCs stained with 143Nd anti-CD5 (UCHT2) and 170Er an... -
APC/Cyanine7 anti-human CD5
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Alexa Fluor® 700 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
PE/Dazzle™ 594 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
TotalSeq™-A0138 anti-human CD5
-
TotalSeq™-C0138 anti-human CD5
-
APC/Fire™ 750 anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
Brilliant Violet 711™ anti-human CD5
Human peripheral blood lymphocytes were stained with CD5 (cl... -
TotalSeq™-B0138 anti-human CD5 Antibody
-
TotalSeq™-D0138 anti-human CD5
-
Brilliant Violet 510™ anti-human CD5
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD5 (Flexi-Fluor™)
-
Brilliant Violet 605™ anti-human CD5
Human peripheral blood lymphocytes were stained with anti-hu...
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