Spark Violet™ 423 anti-mouse H-2Kd/H-2Dd Antibody, H-2Kd/H-2Dd, 34-1-2S

Clone: 34-1-2S (See other available formats)
Regulatory Status: RUO
Other Names: MHC Class I
Isotype: Mouse (C3H) IgG2a, κ
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34-1-2S_SparkViolet423_H-2Kd-H-2Dd_Antibody_1_011024 — C57BL/6 mouse splenocytes were stained with anti-mouse H-2K^d/H-2D^d (clone 34-1-2S) Spark Violet™ 423 (filled histogram). Open histogram represents unstained cells.

Compare all formats See Spark Violet™ 423 spectral data

Cat #	Size	Price	Save
114721	25 µg	¥34,100
114722	100 µg	¥75,900

Description

The 34-1-2S antibody reacts with the H-2K^d/H-2D^d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K^d/H-2D^d haplotype. H-2K^d/H-2D^d is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BDF Splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation², complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
RRID: AB_3097519 (BioLegend Cat. No. 114721)
AB_3097519 (BioLegend Cat. No. 114722)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^d/H-2D^d haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Watts C. 1997. Ann. Rev. Immunol. 15:821.

Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt: View information about H-2Kd/H-2Dd on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All H-2K^d/H-2D^d Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PE anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
Alexa Fluor® 647 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
APC anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PerCP/Cyanine5.5 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PE/Cyanine7 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
APC/Fire™ 750 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
Spark Violet™ 423 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01/10/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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