- Clone
- MECA-79 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PNAd, Peripheral Node Addressin, MECA-79
- Isotype
- Rat IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
120809 | 25 µg | ¥36,300 | |
120810 | 100 µg | ¥98,120 |
MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 β1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymph node stromal cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemistry. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 5 - 10 µg/mL is suggested. For immunohistochemical staining on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm. - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking1-4 of cell adhesion, immunohistochemical staining1,2,6 of acetone-fixed frozen sections and 4% paraformaldehyde-fixed paraffin-embedded sections, flow cytometry10,11, immunoprecipitation3,5, and Western blotting6,11.
-
Application References
(PubMed link indicates BioLegend citation) -
- Streeter PR, et al. 1988. J. Cell Biol. 107:1853. (IHC, Block)
- Michie SA, et al. 1993. Amer. J. Path. 143:1688. (IHC, Block)
- Berg EL, et al. 1991. J. Cell Biol. 114:343. (IP, Block)
- Berg EL, et al. 1993. Nature 366:695. (Block)
- Hemmerich S, et al. 1994. J. Exp. Med. 180:2219. (IP)
- Samulowitz U, et al. 2002. Am. J. Path. 160:1669. (IHC, WB)
- Burke SD, et al. 2007. Diabetes 56:2919. PubMed
- Hirakawa J, et al. 2010. J. Biol. Chem. 285:40864. PubMed
- Thomas SN, et al. 2012. J. Immunol. 189:2181. PubMed.
- Izawa D, et al. 1999. Int. Immunol. 11(12):1989-98. (FC, ICC)
- Sinha RK, et al. 2006. Immunology. 119(4):461-9. (FC, IHC-F, WB)
- RRID
-
AB_2888808 (BioLegend Cat. No. 120809)
AB_2888808 (BioLegend Cat. No. 120810)
Antigen Details
- Structure
- Sulphated CD34, GlyCAM-1 and MAdCAM-1, Sgp200, sialomucin
- Distribution
-
High endothelial venules (HEV) in lymphoid tissues, inflamed tissues and rheumatoid synovia
- Function
- Leukocyte homing, rolling
- Ligand/Receptor
- CD62L
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853.
2. Berg EL, et al. 1993. Nature 366:695.
3. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219.
4. Mitoma J, et al. 2003. J. Biol. Chem. 278:9953.
5. Bruehl RE, et al. 2000. J. Biol. Chem. 275:32642.
6. Bistrup A, et al. 2004. Am. J. Pathol. 164:1635 - Gene ID
- 123803 View all products for this Gene ID 18203 View all products for this Gene ID
- UniProt
- View information about PNAd on UniProt.org
Related FAQs
Other Formats
View All PNAd Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse/human PNAd | MECA-79 | IHC-F,IHC-P,FC,IP,WB |
Biotin anti-mouse/human PNAd | MECA-79 | IHC-F,IHC-P |
Alexa Fluor® 594 anti-mouse/human PNAd | MECA-79 | IHC-P,IHC-F |
Alexa Fluor® 647 anti-mouse/human PNAd | MECA-79 | IHC-P |
Spark YG™ 570 anti-mouse/human PNAd Antibody | MECA-79 | IHC-F,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse/human PNAd
C57BL/6 mouse frozen lymph node section was fixed with 4% pa... Human paraffin-embedded tonsil tissue slices were prepared w... -
Biotin anti-mouse/human PNAd
C57BL/6 mouse frozen lymph node section was fixed with 4% pa... Human paraffin-embedded tonsil tissue slices were prepared w... -
Alexa Fluor® 594 anti-mouse/human PNAd
Human paraffin-embedded tonsil tissue slices were prepared w... C57BL/6 mouse frozen lymph node section was fixed with 4% pa... -
Alexa Fluor® 647 anti-mouse/human PNAd
Human paraffin-embedded tonsil tissue slices were prepared w... -
Spark YG™ 570 anti-mouse/human PNAd Antibody
Human paraffin-embedded tonsil tissue slices were prepared w... C57BL/6 mouse frozen lymph node section was fixed with 4% pa...
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