Spark YG™ 570 anti-Neurofilament H (NF-H), Phosphorylated Antibody, Neurofilament H Phospho (NF-H), SMI 31

Clone: SMI 31 (See other available formats)
Regulatory Status: RUO
Other Names: Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Isotype: Mouse IgG1, κ
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Product Citations: publications

SMI-31_-Spark-YG-570_Neurofilament_Antibody_031023 — IHC staining of Spark YG™ 570 anti-Neurofilament H (NF-H), Phosphorylated (clone SMI 31) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using 1X Retrieve-All Antigen Unmasking #3: Acidic (Cat. No. 927601), the tissue was incubated without (panel -) and with (panel +) Lambda Protein Phosphatase overnight at 4°C, and then incubated without (panel A) or with (panel B) 5.0 µg/mL of Spark YG™ 570 anti-Neurofilament H (NF-H), Phosphorylated (clone SMI 31), overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm

Compare all formats See Spark YG™ 570 spectral data

Cat #	Size	Price	Save
801615	25 µg	¥26,400
801616	100 µg	¥72,600

Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunohistochemical staining on frozen tissue sections, a concentration range of 1.0 - 10.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting¹, immunohistochemistry^2,4, and immunocytochemistry⁴.

SMI 31 reacts with a phosphorylated epitope in extensively phosphorylated neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, which chicken and frog (Xenopus). Immunocytochemically, SMI 31 reacts broadly with thick and thin axons and some dendrites such as basket cell dendrites, but not Purkinje cell dendrites. Nerve cell bodies are generally unreactive. Other cells and tissues are unreactive except for peripheral axons. Phosphatase treatment of tissue sections or Western blots abolishes reaction with SMI 31. Staining is unaffected by trypsin. In pathological conditions, reaction with SMI 31 may be found also in neuronal cell bodies. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures with SMI 31 by agents that induce stress-activated protein kinase. In its reaction with paired helical filaments in hereditary inclusion body myopathy, SMI 31 colocalizes with nitric oxide synthase, suggesting that oxidative stress may play a role in the pathogenic cascade of such degenerative diseases. SMI 31 co-immunoprecipitates neurofilament-associated kinase (NAK 115) via reaction of the antibody with the tail domain of neurofilament H.
RRID: AB_2936763 (BioLegend Cat. No. 801615)
AB_2936763 (BioLegend Cat. No. 801616)

Antigen Details

Structure: Neurofilament H has an apparent molecular mass of 200-220 kD.
Distribution: Tissue distribution: CNS, peripheral nerves and glandular cells of the prostate.
Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion.
Function: Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Interaction: Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.
Cell Type: Mature Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments, Phospho-Proteins
Antigen References: 1. Petzold A. 2005. J. Neurol. Sci. 233 (1-2):183. PubMed
Gene ID: 4744 View all products for this Gene ID
UniProt: View information about Neurofilament H on UniProt.org

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Other Formats

View All Neurofilament H Phospho (NF-H) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Neurofilament H (NF-H), Phosphorylated	SMI 31	IHC-P,WB,ICC
Biotin anti-Neurofilament H (NF-H), Phosphorylated	SMI 31	IHC-P,WB
Alexa Fluor® 594 anti-Neurofilament H (NF-H), Phosphorylated	SMI 31	IHC-P
Alexa Fluor® 488 anti-Neurofilament H (NF-H), Phosphorylated	SMI 31	IHC-P
Alexa Fluor® 647 anti-Neurofilament H (NF-H), Phosphorylated	SMI 31	IHC-P
Spark YG™ 570 anti-Neurofilament H (NF-H), Phosphorylated	SMI 31	IHC-P,IHC-F

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/10/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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