TotalSeq™-D0064 anti-human CD123 antibody, CD123, 6H6

Clone: 6H6 (See other available formats)
Regulatory Status: RUO
Other Names: IL-3Rα, IL-3 Receptor alpha
Isotype: Mouse IgG1, κ
Barcode Sequence: CTTCACTCTGTCAGG
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Product Citations: publications

Cat #	Size	Price	Save
306051	10 µg	¥81,180

Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human IL-3Rα transfected COS cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes: Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting¹, immunoprecipitation¹, and immunohistochemical staining of acetone-fixed frozen sections²and also paraformaldehyde fixed paraffin embedded tissue⁷.
Additional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.
RRID: AB_2892367 (BioLegend Cat. No. 306051)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution: Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
Function: Hematopoietic cell proliferation, differentiation
Ligand/Receptor: IL-3
Cell Type: Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Miyajima A, et al. 1993. Blood 82:1960.
Gene ID: 3563 View all products for this Gene ID
UniProt: View information about CD123 on UniProt.org

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Related Protocols

TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol

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Other Formats

View All CD123 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD123	6H6	FC
PE anti-human CD123	6H6	FC,SB
Purified anti-human CD123	6H6	FC,CyTOF®,IHC-F,IP,WB
PE/Cyanine5 anti-human CD123	6H6	FC
PE/Cyanine7 anti-human CD123	6H6	FC
APC anti-human CD123	6H6	FC
FITC anti-human CD123	6H6	FC
PerCP/Cyanine5.5 anti-human CD123	6H6	FC
Brilliant Violet 421™ anti-human CD123	6H6	FC
Brilliant Violet 650™ anti-human CD123	6H6	FC
Brilliant Violet 510™ anti-human CD123	6H6	FC
Alexa Fluor® 647 anti-human CD123	6H6	FC
Brilliant Violet 605™ anti-human CD123	6H6	FC
Purified anti-human CD123 (Maxpar® Ready)	6H6	FC,CyTOF®
Brilliant Violet 711™ anti-human CD123	6H6	FC
Brilliant Violet 785™ anti-human CD123	6H6	FC
PE/Dazzle™ 594 anti-human CD123	6H6	FC
Alexa Fluor® 488 anti-human CD123	6H6	FC
PE/Cyanine7 anti-human CD123	6H6	FC
TotalSeq™-A0064 anti-human CD123	6H6	PG
Alexa Fluor® 700 anti-human CD123	6H6	FC
APC/Fire™ 750 anti-human CD123	6H6	FC
Pacific Blue™ anti-human CD123	6H6	FC
TotalSeq™-C0064 anti-human CD123	6H6	PG
TotalSeq™-B0064 anti-human CD123	6H6	PG
TotalSeq™-D0064 anti-human CD123	6H6	PG
PerCP anti-human CD123	6H6	FC
GMP PE/Cyanine7 anti-human CD123	6H6	FC
APC/Fire™ 810 anti-human CD123 Antibody	6H6	FC
APC anti-human CD123	6H6	FC
PE anti-human CD123	6H6	FC
Pacific Blue™ anti-human CD123	6H6	FC
Spark Red™ 718 anti-human CD123 (Flexi-Fluor™)	6H6	FC
PerCP anti-human CD123	6H6	FC
PerCP/Cyanine5.5 anti-human CD123	6H6	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.