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- Clone
- G034E3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BN-1, CKR6, DCR2, CKRL3, DRY-6, GPR29, CKR-L3, CMKBR6, GPRCY4, STRL22, GPR-CY4
- Isotype
- Mouse IgG2b, κ
- Barcode Sequence
- GATCCCTTTGTCACT
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CCR6, also known as CD196, is a chemokine receptor that is expressed on immature dendritic cells, B lymphocytes, and memory T cells. CCR6 binds CCL20, although members of the β defensin family also bind CCR6 with a lower affinity. CCR6 positive cells, and its ligand CCL20, have been detected in numerous organs, especially the secondary lymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyers Patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures. In humans, CCR6 can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells resident in both normal and psoriatic skin. CCR6 and/or CCL20 have been implicated in the pathogenesis of rheumatoid arthritis and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 have a role in inflammatory diseases by recruiting Th17 cells to target tissues.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- CCR6-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Additional Product Notes
-
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes. - RRID
-
AB_2894571 (BioLegend Cat. No. 353447)
Antigen Details
- Structure
- Chemokine receptor, GPCR, seven transmembrane receptor
- Distribution
-
Immature dendritic cells, B lymphocytes, memory T cells, Th17 cells
- Function
- Host defense, important for recruitment of B cells to secondary lymphoid organs, mediates arrest of T cells on dermal endothelial cells
- Ligand/Receptor
- CCL20 (MIP-3α)
- Cell Type
- B cells, Dendritic cells, T cells, Th17
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Zaballos A, et al. 1996. Biochem. Bioph. Res. Co. 227:846.
2. Yang D, et al. 1999. Science 286:525.
3. MacDonald KG, et al. 2007. Am. J. Pathol. 170:1229.
4. Homey B, et al. 2000. J. Immunol. 164:6621.
5. Hirota K, et al. 2007. J. Exp. Med. 204:2803.
6. Singh SP, et al. 2008. J. Immunol. 180:214. - Gene ID
- 1235 View all products for this Gene ID
- UniProt
- View information about CD196 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
-
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Other Formats
View All CD196 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with purified CCR6... -
Alexa Fluor® 647 anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 Alexa Fl... 
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Brilliant Violet 421™ anti-human CD196 (CCR6)
Human peripheral blood lymphocytes stained with CD19 PE and ... 
-
PerCP/Cyanine5.5 anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 APC and ... -
PE anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 Alexa Fl... 
-
FITC anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 PE and C... 
-
Alexa Fluor® 488 anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 PE and C... 
-
APC anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 Alexa Fl... 
-
PE/Cyanine7 anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 Alexa Fl... -
Brilliant Violet 605™ anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with CD19 PE... -
Brilliant Violet 785™ anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Brilliant Violet 510™ anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with CD19 AP... 
-
Brilliant Violet 650™ anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 PE and C... 
-
Purified anti-human CD196 (CCR6) (Maxpar® Ready)
Human PBMCs stained with 169Tm-anti-CD19 (HIB19) and 141Pr-a... -
PE/Dazzle™ 594 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with CD19 FI... 
-
APC/Cyanine7 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with CD19 FI... -
Alexa Fluor® 700 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with CD19 FI... 
-
Brilliant Violet 711™ anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with CD19 Al... 
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TotalSeq™-A0143 anti-human CD196 (CCR6)
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Pacific Blue™ anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with CD19 FITC and... -
TotalSeq™-C0143 anti-human CD196 (CCR6)
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APC/Fire™ 750 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with CD19 FI... -
TotalSeq™-B0143 anti-human CD196 (CCR6)
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TotalSeq™-D0143 anti-human CD196 (CCR6)
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PE/Fire™ 640 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark NIR™ 685 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine5 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with anti-hu... -
Biotin anti-human CD196 (CCR6)
Human peripheral lymphocytes were stained with anti-human CD... -
PE/Fire™ 810 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 744 anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human CD196 (CCR6) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS UV395™ anti-human CD196 (CCR6)
Human peripheral blood lymphocytes were stained with anti-hu...
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