TotalSeq™-D0577 anti-human CD73 (Ecto-5'-nucleotidase) antibody, CD73, AD2

Clone: AD2 (See other available formats)
Regulatory Status: RUO
Workshop: V B-CD73.3
Other Names: Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype: Mouse IgG1, κ
Barcode Sequence: CAGTTCCTCAGTTCG
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Product Citations: publications

Cat #	Size	Price	Save
344037	10 µg	¥81,180

Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes: Additional reported applications (for the relevant formats) include:immunofluorescence³.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model⁴.
Additional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.
RRID: AB_2894606 (BioLegend Cat. No. 344037)

Antigen Details

Structure: GPI-linked 5'-nucleotidase, 70 kD
Distribution: Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function: Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.
Gene ID: 4907 View all products for this Gene ID
UniProt: View information about CD73 on UniProt.org

Documentation

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Related Protocols

TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol

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Other Formats

View All CD73 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC,IHC-F
Purified anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC,IHC-F,Block
PE anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
APC anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
PerCP/Cyanine5.5 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Biotin anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
APC/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Brilliant Violet 605™ anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Brilliant Violet 711™ anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Brilliant Violet 785™ anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
TotalSeq™-A0577 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	PG
TotalSeq™-C0577 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	PG
TotalSeq™-B0577 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	PG
APC/Fire™ 750 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
TotalSeq™-D0577 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	PG
Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Alexa Fluor® 647 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Brilliant Violet 510™ anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
PerCP/Fire™ 780 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Spark Red™ 718 anti-hu CD73 (Ecto-5'-nucleotidase) (Flexi-Fluor™)	AD2	FC
PerCP/Fire™ 806 anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC
Spark PLUS B550™ anti-human CD73 (Ecto-5'-nucleotidase)	AD2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/12/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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