- Clone
- N-GAD65 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Glutamate Decarboxylase 2, GAD65, Glutamate Decarboxylase 65 kD Isoform, GABA
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
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844503 | 100 µg |
Item is unavailable |
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GAD gene encodes one of the several forms of glutamic acid decarboxylase, which is identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid (GABA) from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. GAD65 targets presynaptic clusters in axons and exhibits relative dendritic exclusion. GAD65 synthesizes GABA for neurotransmission and is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter (VGAT), which, as a complex, helps package GABA into vesicles for release during neurotransmission.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Peptide immunogen corresponding to amino acids 4-22 of human GAD65.
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry formalin-fixed paraffin-embedded tissues, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation.1
For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844503) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
This antibody demonstrates weaker reactivity to human compared to mouse GAD65/GAD2 -
Application References
(PubMed link indicates BioLegend citation) -
- Hampe CS, et al. 2001. J. Neuroimmunol. 113:63. (IP, WB)
- Hampe CS, et al. 2007. Clin. Exp. Immunol. 148:72.
- Ziegler B, et al. 1996. Acta Diabetol. 33:225.
- Raju R, et al. 2005. J. Immunol. 175:7755.
- RRID
-
AB_2565804 (BioLegend Cat. No. 844503)
Antigen Details
- Structure
- 585 amino acids with a predicted molecular weight of 65 kD.
- Distribution
-
Cytoplasm, cytoplasmic vesicle, golgi apparatus, plasma membrane, and marker of pre-synaptic terminals.
- Function
- Catalyzes production of GABA from L-glutamic acid.
- Interaction
- Taurine and hypotaurine metabolism, transmission across chemical synapses.
- Cell Type
- GABAergic Neurons
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
- Antigen References
-
1. Walls A, et al. 2011. J. of Cerebral Blood Flow & Metabolism 31:494.
- Gene ID
- 2572 View all products for this Gene ID
- UniProt
- View information about Glutamic Acid Decarboxylase on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All Glutamic Acid Decarboxylase (GAD65) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) | N-GAD65 | WB,IHC-P,IP |
Ultra-LEAF™ Purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2) | N-GAD65 | WB,IHC-P,IP |
Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) | N-GAD65 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2)
Western blot of purified anti-Glutamic Acid Decarboxylase (G... IHC staining of purified anti-Glutamic Acid Decarboxylase (G... IHC staining of purified anti-Glutamic Acid Decarboxylase (G... IHC staining of purified anti-Glutamic Acid Decarboxylase (G... -
Ultra-LEAF™ Purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2)
Western blot of purified anti-Glutamic Acid Decarboxylase (G... IHC staining of purified anti-Glutamic Acid Decarboxylase (G... IHC staining of purified anti-Glutamic Acid Decarboxylase (G... IHC staining of purified anti-Glutamic Acid Decarboxylase (G... -
Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2)
IHC staining of Alexa Fluor® 647 anti-Glutamic Acid Deca...
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