- Clone
- A19042E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SIS-delta, T-cell specific protein p288, beta-chemokine RANTES, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine A5
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
620253 | 100 µg | ¥69,720 | |
620254 | 1 mg | ¥331,170 |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CCL5 is a CC chemokine and directs the migration of leukocytes in immune and inflammatory responses. Increased CCL5/RANTES expression has been associated with a wide range of inflammatory disorders and pathologies. This cytokine has been associated as well with the induction or promotion of cancer (prostate and breast). In the latter situation, RANTES can be angiogenic and also increase the metastatic potential of cancer cells by encouraging cellular migration to sites of its production. CCL5/RANTES also inhibits HIV-1 entry and infection by competing with the virus for CCR5 binding.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human CCL5 (RANTES)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the chemotaxis of mouse Baf3- hCCR5 cells induced by recombinant human CCL5 (RANTES) (Cat. No. 580202). ND50 range: 0.04 - 0.3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3068220 (BioLegend Cat. No. 620253)
AB_3068220 (BioLegend Cat. No. 620254)
Antigen Details
- Structure
- Cytokines/Chemokines
- Distribution
-
CCL5 is secreted by lymphocytes, macrophages, eosinophils, platelets, and fibroblasts. RANTES is expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C
- Function
- CCL5 can promote IL-2 and IL-5 expression in human T cells, and the expression of IL-6 and IL-8 in fibroblasts from patients with RA. RANTES modulates the LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation is through the induction of IL-10 by RANTES.
- Interaction
- RANTES/CCL5 is chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils.
- Ligand/Receptor
- CCL5 binds to CCR1, CCR3 and CCR5 receptors. In addition, CCL5 binds to CCR9; nevertheless, it does not induce calcium flux and therefore no chemotactic response.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Schall, et al. 1990. Nature. 347:669-671.
- Cocchi F, et al. 1995. Science. 270:1811-1815.
- Shahrara S, et al. 2006. J. Immunology. 177:5077-5087.
- Larrubia JR, et al. 2008. World J. Gastroenterol. 14:7149-7159.
- Gleissner CA, et al. 2008. Arterioscler Thromb. Vasc. Biol. 28:1920-1927.
- Gene ID
- 6352 View all products for this Gene ID
- UniProt
- View information about CCL5 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CCL5 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Ultra-LEAF™ Purified anti-human CCL5 | A19042E | Neut |
Purified anti-human CCL5 (RANTES) | A19042E | Direct ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human CCL5
Recombinant human CCL5 (RANTES) (Cat. No. 580202) (black cir... -
Purified anti-human CCL5 (RANTES)
Direct ELISA showing purified anti-human CCL5 (clone A19042E...
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