Ultra-LEAF Purified anti-human CD197 CCR7 Antibody, CD197, G043H7

Clone: G043H7 (See other available formats)
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
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Product Citations: publications

Chemotaxis of human CCR7-transfected BaF3 cells was performed in the presence of 15 ng/ml of recombinant CCL19. Addition of LEAF™ purified anti-human CCR7 (clone G043H7) inhibited migration of hCCR7 transfected BaF3 cells (ED 1.42 µg/ml).

Cat #	Size	Price	Save
353255	100 µg	¥39,350
353256	1 mg	¥114,210

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Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC, Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2814290 (BioLegend Cat. No. 353255)
AB_2814290 (BioLegend Cat. No. 353256)

Antigen Details

Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236 View all products for this Gene ID
UniProt: View information about CD197 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD197 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 488 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 421™ anti-human CD197 (CCR7)	G043H7	FC
PE anti-human CD197 (CCR7)	G043H7	FC
APC/Cyanine7 anti-human CD197 (CCR7)	G043H7	FC
Pacific Blue™ anti-human CD197 (CCR7)	G043H7	FC
APC anti-human CD197 (CCR7)	G043H7	FC
FITC anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 647 anti-human CD197 (CCR7)	G043H7	FC
PerCP/Cyanine5.5 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 605™ anti-human CD197 (CCR7)	G043H7	FC
PE/Cyanine7 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 711™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 785™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 510™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 650™ anti-human CD197 (CCR7)	G043H7	FC
PE/Dazzle™ 594 anti-human CD197 (CCR7)	G043H7	FC
Biotin anti-human CD197 (CCR7)	G043H7	FC
Purified anti-human CD197 (CCR7) (Maxpar® Ready)	G043H7	FC,CyTOF®
PerCP anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 700 anti-human CD197 (CCR7)	G043H7	FC
APC/Fire™ 750 anti-human CD197 (CCR7)	G043H7	FC
TotalSeq™-A0148 anti-human CD197 (CCR7)	G043H7	PG
TotalSeq™-B0148 anti-human CD197 (CCR7)	G043H7	PG
TotalSeq™-C0148 anti-human CD197 (CCR7)	G043H7	PG
Brilliant Violet 750™ anti-human CD197 (CCR7)	G043H7	FC
Ultra-LEAF™ Purified anti-human CD197 (CCR7)	G043H7	FC,Block
Spark NIR™ 685 anti-human CD197 (CCR7)	G043H7	FC
KIRAVIA Blue 520™ anti-human CD197 (CCR7)	G043H7	FC
PE/Fire™ 640 anti-human CD197 (CCR7)	G043H7	FC
Spark YG™ 581 anti-human CD197 (CCR7)	G043H7	FC
APC/Fire™ 810 anti-human CD197 (CCR7) Antibody	G043H7	FC
TotalSeq™-D0148 anti-human CD197 (CCR7)	G043H7	PG
PE/Fire™ 810 anti-human CD197 (CCR7) Antibody	G043H7	FC
PE/Cyanine5 anti-human CD197 (CCR7)	G043H7	FC
Spark YG™ 593 anti-human CD197 (CCR7)	G043H7	FC
PE/Fire™ 744 anti-human CD197 (CCR7)	G043H7	FC
Spark Red™ 718 anti-human CD197 (CCR7) (Flexi-Fluor™)	G043H7	FC
Spark Red™ 718 anti-human CD197 (CCR7)	G043H7	FC
PE anti-human CD197	G043H7	FC
Spark PLUS UV395™ anti-human CD197 (CCR7)	G043H7	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 08/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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