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- Clone
- W20238A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKp30, NCR3, Activating NK receptor NKp30, natural cytotoxicity triggering receptor 3
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Biotinylated recombinant human NKp30 (black circles) binds to immobilized recombinant human B7-H6-Fc chimera (Cat. No. 794004). Ultra-LEAF™ purified anti-human CD337 (NKp30) (clone W20238A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have effect. This antibody blocks the binding of 1.2 µg/mL biotinylated human NKp30 to 1 µg/mL immobilized recombinant human B7-H6-Fc chimera. ND50 range: 0.6-3 µg/mL.
CD337, also known as activating NK receptor NKp30 (NKp30), and natural cytotoxicity triggering receptor 3, is recognized by the W20238A monoclonal antibody. CD337, a member of the natural cytotoxicity receptor family is a type I transmembrane protein with one immunoglobulin-like domain and a molecular weight of 30 kD. It exists in six isoforms from alternative splicing. NKp30 is expressed on resting and activated NK cells and enhances NK cell cytolysis of tumor cells that are deficient in MHC class I molecules. CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human NKp30
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by blocking the binding of 1.2 µg/mL recombinant biotinylated human NKp30 to immobilized 1 µg/mL recombinant human B7-H6-Fc chimera (Cat. No. 794004). ND50 range: 0.6 - 3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_3106185 (BioLegend Cat. No. 380605)
Antigen Details
- Structure
- Type I transmembrane protein, member of the natural cytotoxicity receptor family, contains one immunoglobulin-like domain
- Distribution
-
Resting and activated NK cells
- Function
- NK cell triggering of cytolysis toward tumor targets and other target cells deficient in MHC class I molecules
- Interaction
- CD59, TCRζ
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Pende D, et al. 1999. J Exp Med. 190:1505-16.
- Gene ID
- 259197 View all products for this Gene ID
- UniProt
- View information about CD337 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD337 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD337 (NKp30) | W20238A | FC |
| PE anti-human CD337 (NKp30) | W20238A | FC |
| Ultra-LEAF™ Purified anti-human CD337 (NKp30) | W20238A | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD337 (NKp30)
Human peripheral blood lymphocytes were stained with anti-hu... -
Ultra-LEAF™ Purified anti-human CD337 (NKp30)
Biotinylated recombinant human NKp30 (black circles) binds t...
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