Loading...

Ultra-LEAF™ Purified anti-mouse CD269 (BCMA) Antibody

Pricing & Availability
Clone
A16124J (See other available formats)
Regulatory Status
RUO
Other Names
B cell maturation antigen (BCMA), tumor necrosis factor receptor superfamily member 17, CD269
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A16124J_ULEAF_CD269_BCMA_Antibody_042621.png
Biotinylated recombinant mouse TNFRSF17 (black circles) binds to immobilized recombinant mouse TNFSF13. Ultra-LEAF™ purified anti-mouse CD269 (BCMA) antibody (clone A16124J) (purple squares) inhibits the binding in a dose-dependent manner whereas GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.5 µg/mL biotinylated recombinant mouse TNFRSF17 to 0.5 µg/mL of immobilized recombinant mouse TNFSF13. ND50 range: 0.2 - 1.5 µg/mL.
  • A16124J_ULEAF_CD269_BCMA_Antibody_042621.png
    Biotinylated recombinant mouse TNFRSF17 (black circles) binds to immobilized recombinant mouse TNFSF13. Ultra-LEAF™ purified anti-mouse CD269 (BCMA) antibody (clone A16124J) (purple squares) inhibits the binding in a dose-dependent manner whereas GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.5 µg/mL biotinylated recombinant mouse TNFRSF17 to 0.5 µg/mL of immobilized recombinant mouse TNFSF13. ND50 range: 0.2 - 1.5 µg/mL.
Cat # Size Price Save
943603 100 µg ¥53,950
943604 1 mg ¥173,470

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Mouse TNFRSF17-Fc chimera is a type I attached membrane protein of 185 amino acids, which possesses a conserved motif of six cysteines in the N-terminal. It is consistent with the formation of a cysteine repeat motif found in the extracellular domain of TNFRs, and is 62% identical to the human receptor. The overexpression of TNFRSF17-Fc chimera in 293E cells activates NF-κB, Elk-1, the c-Jun N-terminal kinase, and the p38 mitogen-activated protein kinase. After its association with TNF receptor-associated factor (TRAF) 1, TRAF2, and TRAF3. Soluble forms of TACI (TNFRSF13B) and TNFRSF17-Fc chimera bind to BAFF (TNFSFL13B) and APRIL (TNFSFL13A). The interaction of TNFRSF17-Fc chimera to APRIL has higher affinity than the TNFRSF17-BAFF interaction. Analysis of BCMA knockout mice (BCMA−/−) showed no differences in the steady-state frequencies or composition of peripheral B cell subsets (immature, marginal zone, follicular, B1). BCMA expression is upregulated on PBMCs from mice that develop a Sjogren’s-like syndrome, patients with Sjogren’s syndrome, with rheumatoid arthritis, and on B cells from SLE patients. TNFRSF17/BCMA/CD269 is a receptor for TALL-1 and BCMA activates NF-κB through a TRAF5-, TRAF6-, NIK-, and IKK-dependent pathway. The identification of TNFRSF17 as a NF-κB-activating receptor for TALL-1 suggests molecular targets for drug development against certain immunodeficient or autoimmune diseases. Antibody responses to cell-surface BCMA may contribute directly to tumor rejection in vivo.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse TNFRSF17
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of biotinylated recombinant mouse TNFRSF17 at 0.5 µg/mL to immobilized recombinant mouse TNFSF13 at 0.5 µg/mL. ND50 range: 0.2 - 1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892507 (BioLegend Cat. No. 943603)
AB_2892507 (BioLegend Cat. No. 943604)

Antigen Details

Structure
CD Molecules
Distribution

Resting and activated B cells, plasma blasts, plasma cells

Function
B cell maturation factor, maintains the peripheral B cell population and survival of long-lived bone marrow plasma cells, soluble BCMA acts as a decoy receptor for APRIL (TNFSFL13A).
Interaction
Associates with several TRAF family members, monocytes, macrophages, T cells, and dendritic cells
Ligand/Receptor
TNFSF13 (APRIL) and TNFSF (BAFF)
Antigen References
  1. Madry C, et al. 1998. Int. Immunol. 10:1693.
  2. Hatzoglou A, et al. 2000. J. Immunol. 165:1322.
  3. Rennert P, et al. 2000. J. Exp. Med. 192:1677.
  4. Wallweber HJ, et al. 2004. J. Mol. Biol. 343:283.
  5. O'Connor BP, et al. 2004. J. Exp. Med. 199:91.
  6. Kim J, et al. 2011. Autoimmunity. 44:69.
  7. Coquery CM, Erickson LD. 2012. Crit. Rev. Immunol. 32:287.
  8. Laurent SA, et al. 2015. Nature Commun. 6:7333.
Gene ID
21935 View all products for this Gene ID
UniProt
View information about CD269 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account