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Ultra-LEAF™ Purified anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-14 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279, PD 1, PDCD1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMP1-15_UltraLeaf_CD279_Antibody_FC_111014
Con A-stimulated (3 days) C57BL/6 splenocytes were stained with Ultra-LEAF™ purified RMP1-14 (filled histogram) or purified rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • RMP1-15_UltraLeaf_CD279_Antibody_FC_111014
    Con A-stimulated (3 days) C57BL/6 splenocytes were stained with Ultra-LEAF™ purified RMP1-14 (filled histogram) or purified rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
Compare all formats
Cat # Size Price Save
114109 100 µg ¥18,260
114110 1 mg ¥24,740
114119 5 mg ¥89,480
114120 25 mg ¥233,730
114121 50 mg ¥390,770
114122 100 mg ¥561,580

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC, WB, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114108). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 114110) with a lower endotoxin limit than standard LEAF™ purified antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
  2. Personal Communication (WB)
  3. Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
  4. Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
  5. Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  6. Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
  7. Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
  8. Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)
Product Citations
  1. Zhou M, et al. 2020. JCI Insight. :5. PubMed
  2. Godoy–Calderón MJ, et al. 2019. Oncotarget. 4.9625. PubMed
  3. Hardcastle J, et al. 2016. Neuro Oncology. 10.1093/neuonc/now179. PubMed
  4. Dheilly E, et al. 2020. Cancer Cell. 37(5):674-689. PubMed
RRID
AB_2800575 (BioLegend Cat. No. 114109)
AB_2800575 (BioLegend Cat. No. 114110)
AB_2800575 (BioLegend Cat. No. 114119)
AB_2800575 (BioLegend Cat. No. 114120)
AB_2800575 (BioLegend Cat. No. 114121)
AB_2800575 (BioLegend Cat. No. 114122)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 02/17/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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