- Clone
- URA-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MGL, M-ASGP-BP, Macrophage galactose-type C-type lectin
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
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146823 | 100 µg | ¥44,820 | |
146824 | 1 mg | ¥123,340 |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Mouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat. Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92% for the intact sequence and 80% for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2. So far, CD301a has been reported to be involved in recognition and endocytosis of glycoproteins with terminal Gal/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived DCs.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Purified and recombinant mouse MGL2
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_3068151 (BioLegend Cat. No. 146823)
AB_3068151 (BioLegend Cat. No. 146824)
Antigen Details
- Structure
- 42 kD type II transmembrane glycoproteins, contains a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule
- Distribution
-
Dermal dendritic cells
- Function
- Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, tissue remodeling
- Interaction
- Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
- Cell Type
- Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193.
2. Westcott D, et al. 2009. J. Exp. Med. 206:3143.
3. Singh SK, et al. 2009. Mol. Immunol. 46:1240.
4. Sakakura M, et al. 2008. J. Biol. Chem. 283:33665.
5. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892. - Gene ID
- 216864 View all products for this Gene ID
- UniProt
- View information about CD301b on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD301b Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD301b (MGL2) | URA-1 | FC |
PE anti-mouse CD301b (MGL2) | URA-1 | FC |
Alexa Fluor® 647 anti-mouse CD301b (MGL2) | URA-1 | FC |
PE/Cyanine7 anti-mouse CD301b (MGL2) | URA-1 | FC |
PerCP/Cyanine5.5 anti-mouse CD301b (MGL2) | URA-1 | FC |
APC anti-mouse CD301b (MGL2) | URA-1 | FC |
PE/Dazzle™ 594 anti-mouse CD301b (MGL2) | URA-1 | FC |
TotalSeq™-A0566 anti-mouse CD301b (MGL2) | URA-1 | PG |
TotalSeq™-C0566 anti-mouse CD301b (MGL2) | URA-1 | PG |
TotalSeq™-B0566 anti-mouse CD301b (MGL2) Antibody | URA-1 | PG |
Ultra-LEAF™ Purified anti-mouse CD301b (MGL2) | URA-1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD301b (MGL2)
C57BL/6 bone marrow-derived dendritic cells were stained wit... -
PE anti-mouse CD301b (MGL2)
C57BL/6 bone marrow-derived dendritic cells were stained wit... -
Alexa Fluor® 647 anti-mouse CD301b (MGL2)
C57BL/6 bone marrow-derived dendritic cells were stained wit... -
PE/Cyanine7 anti-mouse CD301b (MGL2)
C57BL/6 bone marrow-derived dendritic cells were stained wit... -
PerCP/Cyanine5.5 anti-mouse CD301b (MGL2)
C57BL/6 bone marrow-derived dendritic cells were stained wit... -
APC anti-mouse CD301b (MGL2)
C57BL/6 bone marrow-derived dendritic cells were stained wit... -
PE/Dazzle™ 594 anti-mouse CD301b (MGL2)
C57BL/6 bone marrow-derived dendritic cells were stained wit... -
TotalSeq™-A0566 anti-mouse CD301b (MGL2)
-
TotalSeq™-C0566 anti-mouse CD301b (MGL2)
-
TotalSeq™-B0566 anti-mouse CD301b (MGL2) Antibody
-
Ultra-LEAF™ Purified anti-mouse CD301b (MGL2)
C57BL/6 bone marrow-derived dendritic cells were stained wit...
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