Ultra-LEAF Purified anti-mouse CD335 NKp46 Antibody, CD335, 29A1.4

Clone: 29A1.4 (See other available formats)
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ
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Product Citations: publications

C57BL/6 mouse splenocytes stained with purified 29A1.4 conjugated with PE and NK1.1 (PK136) APC

Cat #	Size	Price	Save
137639	100 µg	¥39,350
137640	1 mg	¥114,210

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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1,2 and in vitro activation of NK cells¹.
RRID: AB_2814037 (BioLegend Cat. No. 137639)
AB_2814037 (BioLegend Cat. No. 137640)

Antigen Details

Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086 View all products for this Gene ID
UniProt: View information about CD335 on UniProt.org

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Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD335 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 510™ anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 711™ anti-mouse CD335 (NKp46)	29A1.4	FC
PE anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Cyanine7 anti-mouse CD335 (NKp46)	29A1.4	FC
Purified anti-mouse CD335 (NKp46)	29A1.4	FC,IHC-F
FITC anti-mouse CD335 (NKp46)	29A1.4	FC
APC anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Cyanine5.5 anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 421™ anti-mouse CD335 (NKp46)	29A1.4	FC
Biotin anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 605™ anti-mouse CD335 (NKp46)	29A1.4	FC
Purified anti-mouse CD335 (NKp46) (Maxpar® Ready)	29A1.4	FC,CyTOF®
Alexa Fluor® 647 anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Dazzle™ 594 anti-mouse CD335 (NKp46)	29A1.4	FC
APC/Fire™ 750 anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 650™ anti-mouse CD335 (NKp46)	29A1.4	FC
TotalSeq™-A0184 anti-mouse CD335 (NKp46)	29A1.4	PG
Brilliant Violet 785™ anti-mouse CD335 (NKp46)	29A1.4	FC
Ultra-LEAF™ Purified anti-mouse CD335 (NKp46)	29A1.4	FC,IHC-F
TotalSeq™-B0184 anti-mouse CD335 (NKp46)	29A1.4	PG
TotalSeq™-C0184 anti-mouse CD335 (NKp46)	29A1.4	PG
APC/Cyanine7 anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Cyanine5 anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Fire™ 806 anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Fire™ 780 anti-mouse CD335 (NKp46)	29A1.4	FC
Spark Red™ 718 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC
Spark Blue™ 574 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC
Spark Blue™ 550 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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