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Ultra-LEAF™ Purified anti-mouse CD90 (Thy1) Antibody

Pricing & Availability
Clone
W18103A (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy1
Isotype
Rat IgG2c, κ
Ave. Rating
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Product Citations
publications
A_W18103A_ULEAF_CD90_Antibody_1_060724
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (clone 145.2c11) APC and Ultra-LEAF™ purifed anti-mouse CD90 (Thy1) (clone W18103A) (left) or purified rat IgG2c, κ isotype control (right) followed by goat anti-rat IgG PE. C57BL/6 mice express the CD90.2 variant of CD90.
  • A_W18103A_ULEAF_CD90_Antibody_1_060724
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (clone 145.2c11) APC and Ultra-LEAF™ purifed anti-mouse CD90 (Thy1) (clone W18103A) (left) or purified rat IgG2c, κ isotype control (right) followed by goat anti-rat IgG PE. C57BL/6 mice express the CD90.2 variant of CD90.
  • B_W18103A_ULEAF_CD90_Antibody_2_060724
    FVB mouse splenocytes were stained with anti-mouse CD3 (clone 145.2c11) APC and Ultra-LEAF™ purifed anti-mouse CD90 (Thy1) (clone W18103A) (left) or purified rat IgG2c, κ isotype control (right) followed by goat anti-rat IgG PE. FVB mice express the CD90.1 variant of CD90.
Cat # Size Price Save
166703 100 µg ¥68,890
Description

CD90, also known as Thy-1, is a 25-35 kD immunoglobulin superfamily member. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells, but not on B cells. CD90 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90 is involved in costimulation of lymphocyte proliferation and activation, and hematopoietic stem cell differentiation. CD90 has been shown to interact with CD45.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W18103A reacts to both the CD90.1 and CD90.2 variants. 

Clone W18103A can reduce CD3+ cell numbers during in vitro incubation with dose-dependent manner.

RRID
AB_3662366 (BioLegend Cat. No. 166703)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neurons, T cells, Thymocytes
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
  2. Craig W, et al. 1993. J Exp Med. 177:1331-42.
  3. Mayani H, et al. 1994. Blood. 83:2410-7.
Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/07/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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