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Ultra-LEAF™ Purified anti-mouse/human CD282 (TLR2) Recombinant Antibody

Pricing & Availability
Clone
QA16A01 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like receptor 2, TLR-2, TIL4, CD282, TLR2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_QA16A01_ULEAF_CD282_Antibody_022717
Human peripheral blood monocytes were stained with purified CD282 (clone QA16A01, filled histogram) or purified mouse IgG1, κ isotype control, followed by anti-mouse IgG PE.
  • 1_QA16A01_ULEAF_CD282_Antibody_022717
    Human peripheral blood monocytes were stained with purified CD282 (clone QA16A01, filled histogram) or purified mouse IgG1, κ isotype control, followed by anti-mouse IgG PE.
  • 2_QA16A01_ULEAF_CD282_Antibody_1_061617

    1 x 105 RAW264.1 cells were cultured in a 96-well plate and starved for 16 hours. Cells were then either left untreated or stimulated with P3CSK4 (100ng/ml), adding isotype control (MOPC-21), parental clone, or recombinant QA16A01. Supernatant was harvested and TNF-α and IL-6 production was determined using BioLegend sandwich ELISAs. Purple bars indicate antibodies at 1µg. Orange bars indicate antibodies at 10µg. Black bars indicate a mean of all unstimulated samples, which includes wells with antibodies at 1 and 10µg but no P3CSK4.

  • 3_QA16A01_ULEAF_CD282_Antibody_2_061617
Compare all formats
Cat # Size Price Save
153001 100 µg ¥43,830
153002 1 mg ¥114,210

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD282 (Toll-like receptor 2, TLR2) is an 85kDa, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular domain and a toll/IL-1R (TIR) in the cytoplasmic region. TLR2 is expressed by monocytes/macrophages, dendritic cells, granulocytes, endothelial cell and epithelial cells. TLR2 associates with TLR1 and TLR6 in the cell surface and is involved in the recognition of pathogen-associated molecular patterns (PAMPs) such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, and LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant extracellular domain of murine TLR2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) Recombinant antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Blocking of IL-6 and TNF-a production induced by P3CSK4 on RAW264.7 cells was observed during development.

Product Citations
  1. Guo Y, et al. 2023. Nat Commun. 7306:14. PubMed
  2. Sung PS, et al. 2019. Nat Commun. 10:2402. PubMed
RRID
AB_2650856 (BioLegend Cat. No. 153001)
AB_2650856 (BioLegend Cat. No. 153002)
Disclaimer

1 x 105 RAW264.1 cells were cultured in a 96-well plate and starved for 16 hours. Cells were then either left untreated or stimulated with P3CSK4 (100mg/ml), adding isotype control (MOPC-21), parental clone, or recombinant QA16A01. Supernatant was harvested and TNF-α and IL-6 production was determined using BioLegend sandwich ELISAs. Purple bars indicate antibodies at 1µg. Orange bars indicate antibodies at 10µg. Black bars indicate a mean of all unstimulated samples, which includes wells with antibodies at 1 and 10µg but no P3CSK4.

Antigen Details

Structure
Type I transmembrane protein, 14 leucine-rich repeats (LRR) in the extracellular domain, the intracellular domain contains a toll/IL-1R (TIR) domain.
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, endothelial cell, epithelial cells.

Function
Activates innate host defense, induces production of pro-inflammatory cytokines.
Interaction
TLR1, TLR6 , MYD88 and TRAF6.
Ligand/Receptor
Peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Biswas S, et al. 2016. Blood. 127:2618.
2. Wang Y, et al. 2016. Proc. Natl. Acad. Sci. USA 113:884.
3. Ramstead AG, et al. 2016. Infect. Immun. 84:940.
4. Scheeren FA, et al. 2014. Nat. Cell. Biol. 16:1238.
5. Barbalat R, et al. 2009. Nat. Immunol. 10:1200. 

Gene ID
24088 View all products for this Gene ID 7097 View all products for this Gene ID
UniProt
View information about CD282 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 0    Revision Date: 03/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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