Ultra-LEAF™ Purified anti-mouse I-A/I-E Antibody, I-A/I-E, M5/114.15.2

Clone: M5/114.15.2 (See other available formats)
Regulatory Status: RUO
Other Names: MHC class II
Isotype: Rat IgG2b, κ
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Product Citations: publications

C57/B6 mouse splenocytes were stained with LEAF™ anti-mouse I-A/I-E (clone M5/114.15.2) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by IgG-FITC.

Cat #	Size	Price	Save
107655	100 µg	¥35,690
107656	1 mg	¥108,730

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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2^b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Activated C57BL/6 mouse spleen cells
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
IHC-F, IP, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A^b, I-A^d, I-A^q, I-E^d, and I-E^k MHC class II alloantigens from mice carrying H-2^p,r,q,b,d,uhaplotypes. Clone M5/114.15.2 however does not react wtih I-A^f, I-A^k, or I-A^s MHC class II alloantigens.¹

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of frozen sections^2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding^4-7, and spatial biology (IBEX)^17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).
RRID: AB_2813968 (BioLegend Cat. No. 107655)
AB_2813968 (BioLegend Cat. No. 107656)

Antigen Details

Structure: MHC class II
Distribution: B cell and activated T cells, APCs of the H-2^b,d,q,r bearing mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt: View information about I-A/I-E on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse I-A/I-E	M5/114.15.2	FC
FITC anti-mouse I-A/I-E	M5/114.15.2	FC
PE anti-mouse I-A/I-E	M5/114.15.2	FC
Purified anti-mouse I-A/I-E	M5/114.15.2	FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E	M5/114.15.2	FC
APC anti-mouse I-A/I-E	M5/114.15.2	FC
Alexa Fluor® 488 anti-mouse I-A/I-E	M5/114.15.2	FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E	M5/114.15.2	FC
Pacific Blue™ anti-mouse I-A/I-E	M5/114.15.2	FC
Alexa Fluor® 700 anti-mouse I-A/I-E	M5/114.15.2	FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E	M5/114.15.2	FC
PerCP anti-mouse I-A/I-E	M5/114.15.2	FC
APC/Cyanine7 anti-mouse I-A/I-E	M5/114.15.2	FC
PE/Cyanine7 anti-mouse I-A/I-E	M5/114.15.2	FC
Brilliant Violet 421™ anti-mouse I-A/I-E	M5/114.15.2	FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E	M5/114.15.2	FC
Purified anti-mouse I-A/I-E (Maxpar® Ready)	M5/114.15.2	FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E	M5/114.15.2	FC
Brilliant Violet 650™ anti-mouse I-A/I-E	M5/114.15.2	FC
Brilliant Violet 711™ anti-mouse I-A/I-E	M5/114.15.2	FC
Brilliant Violet 785™ anti-mouse I-A/I-E	M5/114.15.2	FC
PE/Dazzle™ 594 anti-mouse I-A/I-E	M5/114.15.2	FC
Alexa Fluor® 594 anti-mouse I-A/I-E	M5/114.15.2	IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E	M5/114.15.2	FC
TotalSeq™-A0117 anti-mouse I-A/I-E	M5/114.15.2	PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E	M5/114.15.2	FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E	M5/114.15.2	PG
TotalSeq™-C0117 anti-mouse I-A/I-E	M5/114.15.2	PG
Spark Blue™ 550 anti-mouse I-A/I-E	M5/114.15.2	FC
PE/Fire™ 640 anti-mouse I-A/I-E	M5/114.15.2	FC
Spark YG™ 581 anti-mouse I-A/I-E	M5/114.15.2	FC
PE/Fire™ 810 anti-mouse I-A/I-E	M5/114.15.2	FC
Spark UV™ 387 anti-mouse I-A/I-E	M5/114.15.2	FC
Spark Violet™ 538 anti-mouse I-A/I-E	M5/114.15.2	FC
PerCP/Fire™ 806 anti-mouse I-A/I-E	M5/114.15.2	FC
Spark Red™ 718 anti-mouse I-A/I-E	M5/114.15.2	FC
APC/Fire™ 810 anti-mouse I-A/I-E	M5/114.15.2	FC
Spark PLUS UV395™ anti-mouse I-A/I-E	M5/114.15.2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 07/10/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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