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Ultra-LEAF™ Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
H22 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
H22_021711
Recombinant Mouse IFN-γ was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-mouse IFN-γ antibody (clone H22). Proteins were visualized using a goat anti-Armenian hamster IgG secondary conjugated to HRP and chemiluminescence detection.
  • H22_021711
    Recombinant Mouse IFN-γ was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-mouse IFN-γ antibody (clone H22). Proteins were visualized using a goat anti-Armenian hamster IgG secondary conjugated to HRP and chemiluminescence detection.
Cat # Size Price Save
513207 100 µg ¥18,260
513208 1 mg ¥24,740
513209 5 mg ¥89,480
513210 25 mg ¥233,730
513211 50 mg ¥390,770
513212 100 mg ¥561,580

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Recombinant full-length murine IFN-γ
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
Neut, ELISA, ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified H22 antibody is useful in mouse IFN-γ ELISA, immunofluorescence and immunoprecipitation applications. For in vitro or in vivo neutralization applications, it is suggested to use LEAF™ Purified H22. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schreiber RD, et al. 1985. J. Immunol. 134:1609.
  2. Williams RO, et al. 1993. Clin. Exp. Immunol. 92:323
  3. Steed AL, et al. 2006. J. Virol. 80:192.
  4. Wu LL, et al. 2014. Am J Pathol. 184:2260. PubMed
Product Citations
  1. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
RRID
AB_2810645 (BioLegend Cat. No. 513207)
AB_2810645 (BioLegend Cat. No. 513208)
AB_2810645 (BioLegend Cat. No. 513209)
AB_2810645 (BioLegend Cat. No. 513210)
AB_2810645 (BioLegend Cat. No. 513211)
AB_2810645 (BioLegend Cat. No. 513212)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Function
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571
4. Gray P, et al. 1987. Lymphokines 13:151

Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 01/18/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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