Ultra-LEAF™ Purified anti-mouse IFNAR-1 Antibody, IFNAR-1, MAR1-5A3

Clone: MAR1-5A3 (See other available formats)
Regulatory Status: RUO
Other Names: IFNAR1, IFN-αR1, IFN-α/β Receptor Subunit 1
Isotype: Mouse IgG1, κ
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Product Citations: publications

MAR1-5A3_LEAF_011909 — C57BL/6 splenocytes stained with LEAF™ purified MAR1-5A3, followed by biotinylated anti-mouse IgG and Sav-PE.

Cat #	Size	Price
127321	100 µg	¥18,260
127322	1 mg	¥24,740
127323	5 mg	¥89,480
127324	25 mg	¥233,730
127327	50 mg	¥390,770
127328	100 mg	¥561,580

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

IFNAR-1, the type I IFN receptor subunit 1, is coexpressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs (IFN-α/β). Type I IFNs are a group of structurally and functionally related cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor, and autoimmune responses. Ligand binding to the IFN-α/β receptor complex leads to the tyrosine phosphorylation and activation of IFNAR-1-associated Tyk2 and IFNAR-2-associated Jak1 signal transductions.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Exogenously expressed murine IFNAR1 extracellular domain
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
IP, WB, ELISA, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting^1,2, immunoprecipitation¹, ELISA¹, and blocking^8,9. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127322) (Endotoxin <0.01 EU/µg).
RRID: AB_11150409 (BioLegend Cat. No. 127321)
AB_11150409 (BioLegend Cat. No. 127322)
AB_11150409 (BioLegend Cat. No. 127323)
AB_11150409 (BioLegend Cat. No. 127324)
AB_11150409 (BioLegend Cat. No. 127327)
AB_11150409 (BioLegend Cat. No. 127328)

Antigen Details

Distribution: IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
Ligand/Receptor: IFN-α/β
Antigen References: 1. Branca AA, et al. 1981. Nature 294:768.
2. Orchansky P, et al. 1984. J. Interferon Res. 4:275.
3. Hemmi S, et al. 1994. Cell 76:803.
4. Novick D, et al. 1994. Cell 77:391.
Gene ID: 15975 View all products for this Gene ID
UniProt: View information about IFNAR-1 on UniProt.org

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Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All IFNAR-1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse IFNAR-1	MAR1-5A3	FC,IP,WB,ELISA,Block
Biotin anti-mouse IFNAR-1	MAR1-5A3	FC,IP,WB,ELISA
PE anti-mouse IFNAR-1	MAR1-5A3	FC
APC anti-mouse IFNAR-1	MAR1-5A3	FC
Ultra-LEAF™ Purified anti-mouse IFNAR-1	MAR1-5A3	FC,IP,WB,ELISA,Block
PE/Cyanine7 anti-mouse IFNAR-1	MAR1-5A3	FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 08/16/2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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