Ultra-LEAF Purified anti-mouse Ly49H Antibody, Ly49H, 3D10

Clone: 3D10 (See other available formats)
Regulatory Status: RUO
Other Names: Klra8, Cmv1
Isotype: Mouse IgG1, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with CD49b (DX5) Pacific Blue™ and purified Ly49H (clone 3D10, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 FITC.

Cat #	Size	Price	Save
144717	100 µg	¥44,820
144718	1 mg	¥112,390

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Description

Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein. It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice). Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice. Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection. Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C1498-Ly49A/H transfectant
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats of this clone) include: immunoprecipation¹, and in vitro² and in vivo³ blocking activity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 & 144718).
RRID: AB_2819889 (BioLegend Cat. No. 144717)
AB_2819889 (BioLegend Cat. No. 144718)

Antigen Details

Structure: 28.3 kD
Distribution: NK cells but not NKT cells
Function: Interacts with m157, a cytomegalovirus-encoded protein
Ligand/Receptor: m157
Cell Type: NK cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.
Gene ID: 16639 View all products for this Gene ID
UniProt: View information about Ly49H on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All Ly49H Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse Ly49H	3D10	FC
Purified anti-mouse Ly49H	3D10	FC,IP,Block
PE anti-mouse Ly49H	3D10	FC
Alexa Fluor® 647 anti-mouse Ly49H	3D10	FC
PE/Cyanine7 anti-mouse Ly49H	3D10	FC
APC anti-mouse Ly49H	3D10	FC
TotalSeq™-A0839 anti-mouse Ly49H	3D10	PG
Ultra-LEAF™ Purified anti-mouse Ly49H	3D10	FC,IP,Block
TotalSeq™-C0839 anti-mouse Ly49H	3D10	PG
TotalSeq™-B0839 anti-mouse Ly49H	3D10	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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