Veri-Cells™ Activated (Cytokine) PBMC

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RUO
Other Names
Control Cells
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VeriCells_Acivated_Cytokine_PBMC_1_102618
Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and TNF-α PerCP/Cyanine5.5, or GM-CSF Pacific Blue™ (left). Plots were gated based on corresponding isotype control stains (right).
  • VeriCells_Acivated_Cytokine_PBMC_1_102618
    Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and TNF-α PerCP/Cyanine5.5, or GM-CSF Pacific Blue™ (left). Plots were gated based on corresponding isotype control stains (right).
  • VeriCells_Acivated_Cytokine_PBMC_2_102618
    Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and IL-17a Brilliant Violet™ 421, or IFNγ APC (left). Plots were gated based on corresponding isotype control stains (right).
  • VeriCells_Acivated_Cytokine_PBMC_3_091524
    Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and IL-2 APC/Cyanine7, or IL-4 PE/Cyanine7 (left). Plots were gated based on corresponding isotype control stains (right).
  • VeriCells_Acivated_Cytokine_PBMC_4_102618
    Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and IL-8 APC, or IL-13 APC (left). Plots were gated based on corresponding isotype control stains (right).
Cat # Size Price Save
427001 10 tests ¥32,340
427003 50 tests ¥116,160
Description

The reconstituted Veri-Cells™ Activated (Cytokine) PBMC can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as controls for cytokine staining with minimal variability across experiments. These cells have been verified to work with commonly tested cell surface (CD3, CD4, CD8, CD19, CD56) as well as cytokine markers such as IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17, GM-CSF, IFN gamma, and TNF alpha. Some targets such as IL-5 are rare and will require the acquisition of at least 100,000 CD3+ events.

This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus using FDA approved methods. Biological tests are not 100% accurate. Use standard precautions when handling, and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.

Product Details
Technical data sheet

Kit Contents

Kit Contents

Cat. No. 427001

  • 2 vials of Veri-Cell™ Activated (Cytokine) PBMC (≥1 X 106 cells per vial) 
  • 1 vial of 0.7 mL Veri-Cells™ Buffer A Plus

Cat. No. 427003

  • 10 vials of Veri-Cell™ Activated (Cytokine) PBMC (≥1 X 106 cells/vial)
  • 5 vials of 0.7 mL Veri-Cells™ Buffer A Plus
     

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Product Details

Formulation
Cat. No. 427001: 2 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Cat. No. 427003: 10 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Preparation
The Veri-Cells™ Activated (Cytokine) PBMC kit is prepared from 2 vials of lyophilized human activated PBMCs and a vial of Veri-Cells™ Buffer A Plus. Veri-Cells Activated (Cytokine) PBMC contain lyophilized PBMCs that had been stimulated using Cell Activation Cocktail with Brefeldin A (Cat. No. 423303) for 6 hours.
Storage & Handling
Store the kit at 2°C – 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C – 8°C in the buffer provided.
Application

FC - Quality tested

Recommended Usage

50 µL per test (after reconstitution with 325 µl of Veri-Cells™ Buffer A Plus)

Once reconstituted, let the cells sit for 15 minutes at room temperature prior to staining.

Veri-Cells™ Buffer A Plus may show precipitation over time, however this is normal and does not affect performance of the buffer.

Application Notes

Veri-Cells™ Activated (Cytokine) PBMC Kit can be used as a positive control for detecting common surface (CD3, CD4, CD8, CD19, CD56) as well as cytokine markers such as IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17a, GM-CSF, IFN gamma, and TNF alpha. Assessing the suitability of using this product as a control for end user’s molecule of interest is recommended, as each lot is verified based on the expression levels of markers mentioned above. For best results, Cell Staining Buffer is recommended for any washing steps. Product performance specifications are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.

Antibodies Used for Product Validation

Target

Clone

Fluorophore

CD3

SK7

FITC

IL-13

JES10-5A2

PE/Cyanine7

GM-CSF

BVD2-21C11

Pacific Blue™

TNF-alpha

Mab11

PerCP/Cyanine5.5

IL-2

MQ1-17H12

APC/Cyanine7

IL-8

E8N1

APC

IL-17a

BL168

Brilliant Violet 421™

IFN-gamma

4S.B3

APC

IL-5

TRFK5

APC

IL-4

MP4-25D2

PE/Cyanine7

IL-3

BVD3-1F9

PE

 

Cell subset

Acceptance Criteria, % positive of lymphocyte gate

CD3+IL-2+

10-40

CD3+IL-3+

0.2-5

CD3+ IL-4+

0.1-3

CD3+ IL-5+

0.2-3

CD3+IL-8+

0.1-5

CD3+IL-13+

0.2-5

CD3+IL-17A+

0.1-2

CD3+IFN gamma+

5-30

CD3+TNF alpha+

10-60

CD3+GM-CSF+

1-15

Note on indicated ranges: The antibodies used for testing were the following: CD3 APC/Cyanine7 (clone SK7), IL-2 APC/Cyanine7 (clone MQ1-17H12), IL-3 PE (clone BVD3-1F9), IL-4 PE/Cyanine7 (clone MP4-25D2), IL-5 APC (clone TRFK5), IL-8 PE/Cyanine7 (clone E8N1), IL-13 APC (clone JES10-5A2), IL-17a Brilliant Violet™ 421 (clone BL168), GM-CSF Pacific Blue™ (clone BVD2-21C11), TNF-α PerCP/Cyanine5.5 (clone MAB11), and IFN-ʎ APC (clone 4S.B3). Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. The Certificate of Analysis specific values are provided as a reference. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ Activated (Cytokine) PBMC. 

Additional Product Notes

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
NA

Related FAQs

Is washing required after staining?

Yes, it is recommended to wash after staining. We recommend using BioLegend's Cell Staining buffer (Cat. No. 420201) for the wash step.

Can the cells be activated after rehydration?
No, they cannot be activated.
Are these cells virus/pathogen free?
These are tested to be free of HIV, HBV, syphilis, and HCV.
Are there any RBCs in Veri-cells™?
There may be a few, but the majority of RBCs are not present in Veri-Cells™ preparations.
Are these cells viable?
No they are not.
Can a constant percentage of each population be expected in each lot?
Yes, within each lot, cell populations remain constant.
Are the cells fixed?

They are treated with our proprietary mixture prior to lyophilization.

Can they be used to detect intracellular cytokines such as TNF-α or IFN-γ?

For this, use Veri-Cells™ Activated (Cytokine) PBMC which have been activated with Cell Activation Cocktail (with Brefeldin A) containing PMA and Ionomycin. These have been validated to stain positive for activation-induced cytokines including TNF-α and IFN-γ. Veri-Cells™ PBMC and Veri-Cells™ CD4-Low PBMC will not have detectable TNF-α or IFN-γ.

How do we QC these cells?

Each Veri-Cells™ product is tested with a panel of antibodies to ensure expression of these markers falls within the expected range.  For more information regarding the exact markers used for each product, please contact technical service.

Go To Top Version: 5    Revision Date: 09/17/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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