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- Regulatory Status
- RUO
- Other Names
- Syndecan-1, Control cells
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Veri-Cells™ CD138 Leukocytes were stained with anti-human CD45 (clone 2D1) PE, anti-human CD19 (clone SJ25C1) APC, anti-human CD38 (clone HIT2) FITC, and anti-human CD138 (clone MI15) Brilliant Violet 421™. CD45- and CD19-negative cells (left plot) were analyzed for CD138 and CD38 expression (right plot). Gates for the CD38 vs CD138 plot were set based on corresponding isotype controls. -
Veri-Cells™ CD138 Leukocytes were stained with Human TBNK 6 Color Cocktail (Cat. No. 391503). Lymphocytes (left plot) were used for analysis of CD45-positive CD3-positive and CD45-positive CD3-negative populations (right plot). -
Veri-Cells™ CD138 Leukocytes were stained with Human TBNK 6 Color Cocktail (Cat. No. 391503). CD45-positive CD3-positive (left plot) and CD45-positive CD3-negative (right plot). populations are shown.
The reconstituted Veri-Cells™ CD138 Leukocytes can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as controls for stability studies to avoid donor variability. Veri-Cells™ CD138 Leukocytes contain a mixture of both CD138-postive and CD138-negative cells. The CD19- CD45- CD138+ population co-expresses CD38, CD56, and CD269. In addition to these, the leukocyte population in this product has been verified to work with commonly tested cell markers such as CD3, CD4, CD8, CD19, CD20, CD56/16, CD14, CD25, CD69, HLA-DR, Granzyme B, Perforin, Foxp3, and Helios.
This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative, using FDA approved methods, for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C viruses and HTLV (Human T-Lymphotropic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis, and West Nile Virus. Biological tests are not 100% accurate. Use standard precautions when handling and treat as if the product is capable of transmitting diseases. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.
Kit Contents
- Kit Contents
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Cat. No. 427801
- 2 vials of lyophilized Veri-Cells™ CD138 Leukocytes (≥ 1 x 106 cells per vial)
- 1 vial containing 0.7 mL of Veri-Cells™ Buffer B
Product Details
- Formulation
- 2 vials each containing ≥ 1 X 106 lyophilized human peripheral blood leukocyte and 1 vial of 0.7 mL Buffer B.
- Preparation
- The Veri-Cells™ CD138 Leukocytes kit is a mixture of KMS-26 cells and human leukocytes.
- Concentration
- 1 X 106 cells per vial (lyophilized)
- Storage & Handling
- Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
- Application
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FC - Quality tested
- Recommended Usage
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50 µL per test (after reconstitution with 325 µL of Veri-Cells™ Buffer B).
Once reconstituted, let the cells sit for 15 minutes at room temperature prior to staining.
Veri-Cells™ Buffer B may show precipitation over time, however this is normal and does not affect performance of the buffer. - Application Notes
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The Veri-Cells™ CD138 Leukocytes kit can be used as a staining control that provides assay values for CD38 and CD138. For best results, Cell Staining Buffer (Cat. No. 420201) is recommended for any washing steps. Please note, any claims relative to product performance are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.
Cell subset
Acceptance Criteria
CD38+CD138+
40%-100%
Note on indicated ranges: Percent positive values are based on gates set on isotype controls. The antibodies used for testing were the following: CD45 PE (clone 2D1) and CD19 APC (clone SJ25C1), CD38 FITC (clone HIT2), and CD138 Brilliant Violet 421™ (clone MI15). It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.
Please use our CoA Look-Up Tool to find the expected frequencies of CD38+/CD138+ cells for each lot as determined by analysis of CD38 FITC (clone HIT2) and CD138 Brilliant Violet 421™ (clone MI15) positive single cells. The values reported on the Certificate of Analysis are provided as a reference only. Variability in antibodies used, instruments, staining conditions, and other factors may influence the end user values. - Additional Product Notes
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Note: Buffer A and Buffer A Plus cannot be used interchangeably with Buffer B.
Antigen Details
- Molecular Family
- CD Molecules
- Gene ID
- NA
Related FAQs
- Is washing required after staining?
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Yes, it is recommended to wash after staining. We recommend using BioLegend's Cell Staining buffer (Cat. No. 420201) for the wash step.
- Can the cells be activated after rehydration?
- No, they cannot be activated.
- Are these cells virus/pathogen free?
- These are tested to be free of HIV, HBV, syphilis, and HCV.
- Are there any RBCs in Veri-cells™?
- There may be a few, but the majority of RBCs are not present in Veri-Cells™ preparations.
- Are these cells viable?
- No they are not.
- Can a constant percentage of each population be expected in each lot?
- Yes, within each lot, cell populations remain constant.
- Are the cells fixed?
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They are treated with our proprietary mixture prior to lyophilization.
- Can they be used to detect intracellular cytokines such as TNF-α or IFN-γ?
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For this, use Veri-Cells™ Activated (Cytokine) PBMC which have been activated with Cell Activation Cocktail (with Brefeldin A) containing PMA and Ionomycin. These have been validated to stain positive for activation-induced cytokines including TNF-α and IFN-γ. Veri-Cells™ PBMC and Veri-Cells™ CD4-Low PBMC will not have detectable TNF-α or IFN-γ.
- How do we QC these cells?
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Each Veri-Cells™ product is tested with a panel of antibodies to ensure expression of these markers falls within the expected range. For more information regarding the exact markers used for each product, please contact technical service.
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