Loading...

APC Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Allophycocyanin, SAv-APC
Ave. Rating
Submit a Review
Product Citations
publications
APC_Streptavidin_Antibody_042123
Human peripheral blood lymphocytes were stained with APC streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (APC) Streptavidin (filled histogram).
  • APC_Streptavidin_Antibody_042123
    Human peripheral blood lymphocytes were stained with APC streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (APC) Streptavidin (filled histogram).
See APC spectral data
Cat # Size Price Save
405207 100 µg ¥26,180
Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-APC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-APC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC is 320 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Kamimura D, et al. 2006. J. Immunol. 177:306.
  2. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
  3. Chou WC,et al.2006.Blood108:3005. PubMed
  4. Malagolini N, et al. 2009. Glycobiology. 19:172. PubMed
  5. Kroeger KM, et al. 2009. J. Leukocyte Biol. PubMed
  6. Imada M, et al. 2009. Int. Immunol. 21:1151. PubMed
  7. Yi Y, et al. 2010 J. Biol Chem. 285:21233. PubMed
  8. King IL, et al. 2010. J. Immunol. 185:6138. PubMed
  9. Yokoyama T, et al. 2011. Int Immunol. PubMed
  10. Rusert P, et al. 2011. J. Exp Med. 208:1419. PubMed
  11. Bollig N, et al. 2012. PNAS. 109:8664. PubMed
  12. Nguyen V, et al. 2012. J. Immunol. 189:1081. PubMed
  13. Jager NA, et al. 2012. J Nucl Med. 53:1229. PubMed
  14. Liberman AC, et al. 2012. Mol Immunol. 50:220. PubMed
  15. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
  16. Ludin A, et al.2012. Nat Immunol. 13:1072. PubMed
  17. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  18. Tani-Ichi S, et al. 2012. PNAS. 110:612. PubMed
  19. Jin L, et al. 2013. PNAS. 110:3907. PubMed
  20. Ota T, et al. 2013. J. Immunol. 191:3179. PubMed
  21. Fu X, et al. 2013. PNAS. 110:17982. PubMed
  22. Ryan SO, et al. 2014. Glycobiology. 24:362. PubMed
  23. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  24. Pilling D, et al. 2014. PLoS One. 9:93730. PubMed
  25. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
  26. Owen KA, et al. 2014. PLoS Pathog. 10:1004159. PubMed
  27. Duek A, et al. 2014. Blood. 123:3943. PubMed
  28. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  29. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
  30. Lundberg P, et al. 2014. J Exp Med. PubMed
  31. Martin RK, et al. 2014. PLoS One. 9:110609. PubMed
  32. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
  33. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
  34. Jager NA, et al. 2014. J Nucl Med. 55:1945. PubMed
  35. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  36. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  37. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  38. Kim Yu, et al. 2015. PLoS One. 10:120294. PubMed
  39. Zhang L, et al. 2015. J Immunol. 194:5243. PubMed
Product Citations
  1. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  2. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
  3. Preece R, et al. 2020. Gene Ther. 451:27. PubMed
  4. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  5. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  6. Cohen–Khait R, et al. 2017. Structure. 25:1867. PubMed
  7. Goel RR, et al. 2022. Cell. 185:1875. PubMed
  8. Sugimoto M, et al. 2022. DNA Res. 29: . PubMed
  9. Nutalai R, et al. 2022. Cell. 185:2116. PubMed
  10. Singh S, et al. 2022. iScience. 25:104549. PubMed
  11. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  12. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  13. Cao Y, et al. 2023. Nature. 614:521. PubMed
  14. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  15. Hernandez SPA, et al. 2023. Front Immunol. 13:1035344. PubMed
  16. Pušnik J, et al. 2023. Nat Commun. 14:572. PubMed
  17. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  18. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  19. Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed
  20. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  21. White Z, et al. 2022. Blood Cells Mol Dis. 97:102688. PubMed
  22. Chen HY, et al. 2022. Elife. 11:. PubMed
  23. Parry H, et al. 2022. Cell Rep Med. 3:100739. PubMed
  24. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  25. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  26. Koutsakos M, et al. 2023. Immunity. 56:879. PubMed
  27. García-Vega M, et al. 2023. iScience. 26:106562. PubMed
  28. Liu Y, et al. 2023. Nat Commun. 14:2179. PubMed
  29. Hie B, et al. 2023. Nat Biotechnol. . PubMed
  30. Li X, et al. 2023. Nat Commun. 14:2929. PubMed
  31. Lan Y, et al. 2023. iScience. 26:106817. PubMed
  32. Malagolini N, et al. 2009. Glycobiology. 0.911111111. PubMed
  33. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  34. Schneider C, et al. 2018. Cell. 174:271. PubMed
  35. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  36. Tarban N, et al. 2022. Cells. 11:. PubMed
  37. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  38. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  39. Georgiadis C, et al. 2021. Leukemia. . PubMed
  40. Ota T, et al. 2013. J Immunol. 191:3179. PubMed
  41. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
  42. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  43. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  44. Pazina T, et al. 2019. Cancer Immunol Res. 7:1633. PubMed
  45. Temming AR, et al. 2021. Front Immunol. 12:594773. PubMed
  46. Blake SJ, et al. 2021. Cell Rep Med. 2:100464. PubMed
  47. Sarkar R, et al. 2021. Bio Protoc. 11:e4255. PubMed
  48. Hou S, et al. 2021. Sci Immunol. 6:eabg9433. PubMed
  49. Yamanaka S, et al. 2021. STAR Protocols. 2(1):100314. PubMed
  50. J L, et al. 2016. Sci Rep. 6:30904. PubMed
  51. Gaudreault V, et al. 2015. PLoS One. 10: e0140805. PubMed
  52. Jager N, et al. 2014. J Nucl Med. 55:1945. PubMed
  53. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
  54. Jager N, et al. 2012. J Nucl Med. 53:1222. PubMed
  55. Kimura T, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L271. PubMed
  56. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  57. Lebert-Ghali C, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.03.003. PubMed
  58. da Mota JB, et al. 2020. J Leukoc Biol. 108:1239. PubMed
  59. Niewolik D, et al. 2022. Nucleic Acids Res. 50:2096. PubMed
  60. Io S, et al. 2021. STAR Protoc. 2:100921. PubMed
  61. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  62. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  63. Jin L, et al. 2016. Stem Cell Res. 16:40-53. PubMed
  64. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  65. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  66. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  67. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
  68. Rusert P, et al. 2011. J Exp Med. 208:1419. PubMed
  69. Barcala Tabarrozzi A, et al. 2016. Sci Rep. 6:36646. PubMed
  70. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  71. Liu C, et al. 2022. Cell Host Microbe. 30:53. PubMed
  72. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  73. Cao Y, et al. 2022. Nature. :. PubMed
  74. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  75. Toulmin SA, et al. 2021. Nat Commun. 12:3993. PubMed
  76. Duek A, et al. 2014. Blood. 123:3943. PubMed
  77. Yokoyama T, et al. 2011. J Immunol. 208:885. PubMed
  78. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  79. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  80. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  81. Bollig N, et al. 2012. Proc Natl Acad Sci U S A. 109:8664. PubMed
  82. Yi Y, et al. 2010. J Biol Chem. 285:21233. PubMed
  83. Chou W, et al. 2006. Blood. 108:3005. PubMed
  84. Tashita C, et al. 2020. World J Gastroenterol. 26:918. PubMed
  85. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  86. Cao Y, et al. 2022. Nature. 602:657. PubMed
  87. Kakizaki M, et al. 2021. Cell Death Dis. 12:1010. PubMed
  88. Tani-ichi S, et al. 2012. Proc Natl Acad Sci U S A. 110:612. PubMed
  89. Ryan S, et al. 2014. Glycobiology. 24:262. PubMed
  90. Han X, et al. 2017. Mol Ther. 25(1):274-284. PubMed
  91. Hao Y, et al. 2020. J Biol Chem. . PubMed
  92. Parikh BA, et al. 2020. Cell Rep. 32:107969. PubMed
  93. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  94. Wang L, et al. 2022. Nat Commun. 13:4709. PubMed
  95. Kunitomi A, et al. 2022. Cell Rep Methods. 2:100317. PubMed
  96. Mizoguchi I, et al. 2020. J Clin Invest. 130:6124. PubMed
  97. Locke F, et al. 2017. Mol Ther. 25(1):285-295. PubMed
  98. Jennings R, et al. 2016. Int Immunol. 28: 503 - 512. PubMed
  99. Morita N, et al. 2016. Innate Immunity. 10.1177/1753425916672312. PubMed
  100. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
  101. Owen K, et al. 2014. PLoS Pathog. 10:1004159. PubMed
  102. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  103. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  104. Nguyen V, et al. 2012. J Immunol. 189: 1081. PubMed
  105. Jin L, et al. 2013. Proc Natl Acad Sci U S A. 110:3907. PubMed
  106. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  107. Audemard-Verger A, et al. 2017. J Immunol. 10.4049/jimmunol.1700430. PubMed
  108. Hermann BP, et al. 2018. Cell Rep. 25:1650. PubMed
  109. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  110. Friedrich SK, et al. 2021. Pathogens. 10: . PubMed
  111. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  112. Mainini F, et al. 2015. PLoS One. 10: 0145403. PubMed
  113. Zhang L, et al. 2015. J Immunol. 194:5423. PubMed
  114. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
  115. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
  116. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  117. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
  118. Pascual G, et al. 2021. Nature. 599:485. PubMed
  119. Saini SK, et al. 2021. Sci Immunol. 6:. PubMed
  120. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
  121. Dejnirattisai W, et al. 2021. Cell. 2183:184. PubMed
  122. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  123. Jiang H, et al. 2011. PLoS One. 6:e27464. PubMed
  124. Collins DR, et al. 2021. Immunity. 54:2372. PubMed
  125. Larcombe-Young D, et al. 2022. STAR Protoc. 3:101414. PubMed
  126. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  127. Lundberg P, et al. 2014. J Exp Med. 211:2213. PubMed
  128. King I, et al. 2010. J Immunol. 185:6138. PubMed
  129. Tuekprakhon A, et al. 2022. Cell. 185:2422. PubMed
  130. Kim SP, et al. 2022. Cancer Immunol Res. :OF1. PubMed
  131. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  132. Martin R, et al. 2014. PLoS One. 9:110609. PubMed
  133. Budai Z, et al. 2021. Cells. 10:. PubMed
  134. Bainter W, et al. 2021. Sci Immunol. 6:eabf6723. PubMed
  135. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  136. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  137. Koide S, et al. 2022. iScience. 25:103603. PubMed
  138. Kosti P, et al. 2021. STAR Protocols. 2:100723. PubMed
  139. Yewdell WT, et al. 2021. Cell Rep. 37:109961. PubMed
  140. Nguyen NT, et al. 2021. Nat Nanotechnol. 16:1424. PubMed
  141. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  142. Mazor Y, et al. 2017. Sci Rep. 7:40098. PubMed
  143. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  144. Liberman A, et al. 2012. Mol Immunol. 50:220. PubMed
  145. GR N, et al. 2012. PLoS One. 7:e39045. PubMed
  146. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  147. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  148. Tripathi S, et al. 2016. Proc Natl Acad Sci U S A. 113: 1555 - 1564. PubMed
  149. Hauser J, et al. 2016. Mol Immunol. 80:78-90. PubMed
  150. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  151. Pedersen NW, et al. 2019. Nat Commun. 10:837. PubMed
  152. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  153. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  154. Kroeger K, et al. 2009. J Leukoc Biol. 86:769. PubMed
  155. Zahradník J, et al. 2021. ACS Synth Biol. Online ahead of print. PubMed
  156. Chen Y, et al. 2021. Cancer Res Treat. Online ahead of print. PubMed
  157. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  158. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  159. Tagawa M, et al. 2016. PLoS One. 11: 0150030. PubMed
  160. Fu X, et al. 2013. Proc Natl Acad Sci U S A. 110:17892. PubMed
  161. Fichtner ML, et al. 2022. Acta Neuropathol Commun. 10:154. PubMed
  162. Holm JS, et al. 2022. Nat Commun. 13:1935. PubMed
Go To Top Version: 5    Revision Date: 04/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account