Direct-Blot HRP anti-beta-actin Antibody, beta-actin, W16197A

Clone: W16197A (See other available formats)
Regulatory Status: RUO
Other Names: ACTB, Actin, cytoplasmic 1, Beta-actin
Isotype: Rat IgG2a, κ
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Product Citations: publications

W16197A_DBHRP_beta-actin_Antibody_032918 — Whole cell lysates (15 µg protein) from HeLa was resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:10,000 diluted (0.01 µg/mL), 1:40,000 diluted (0.0025 µg/mL),1:100,000 diluted (0.001 µg/mL) Direct-Blot™ HRP anti-β-actin Antibody, clone W16197A, and 1:10,000 diluted (0.01 µg/mL) purified anti-β-actin Antibody, clone W16197A (Cat. No. 664801) (upper). Proteins were visualized using an 1:3000 diluted HRP anti rat-IgG secondary antibody (Cat. No.405405) for purified anti-β-actin Antibody and chemiluminescence detection. Ponceau S staining was used as loading control (lower). Lane M: MW ladder.

Cat #	Size	Price	Save
664803	25 µL	¥22,220
664804	100 µL	¥74,800

Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat, Zebrafish, Drosophila
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human ACTB peptide (1-13 a.a.).
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml (1:10,000 - 1:100,000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: Reactivity to Drosophila was only verified with the purified format.
RRID: AB_2728496 (BioLegend Cat. No. 664803)
AB_2728496 (BioLegend Cat. No. 664804)

Antigen Details

Structure: 375 amino acids with a predicted molecular weight of 42 kD.
Distribution: Cytoplasm
Function: Actins are highly conserved proteins that are involved in cell motility.
Interaction: Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microfilaments
Antigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
Gene ID: 60 View all products for this Gene ID
UniProt: View information about beta-actin on UniProt.org

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Other Formats

View All β-actin Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-β-actin	W16197A	WB
Direct-Blot™ HRP anti-β-actin	W16197A	WB

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03/29/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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